지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 995 - 1,001 (7page)
- DOI
- 10.1007/s13258-021-01105-z
이용수
초록· 키워드
오류제보하기
Background Tumor-treating felds (TTFields) is an emerging non-invasive cancer-treatment modality using alternating electric felds with low intensities and an intermediate range of frequency. TTFields afects an extensive range of charged and polarizable cellular factors known to be involved in cell division. However, it causes side-efects, such as DNA damage and apoptosis, in healthy cells.
Objective To investigate whether thymidine can have an efect on the DNA damage and apoptosis, we arrested the cell cycle of human glioblastoma cells (U373) at G1/S phase by using thymidine and then exposed these cells to TTFields.
Methods Cancer cell lines and normal cell (HaCaT) were arrested by thymidine double block method. Cells were seeded into the gap of between the insulated wires. The exposed in alternative electric felds at 120 kHz, 1.2 V/cm. They were counted the cell numbers and analyzed for cancer malignant such as colony formation, Annexin V/PI staining, γH2AX and RT-PCR.
Results The colony-forming ability and DNA damage of the control cells without thymidine treatment were signifcantly decreased, and the expression levels of BRCA1, PCNA, CDC25C, and MAD2 were distinctly increased. Interestingly, however, cells treated with thymidine did not change the colony formation, apoptosis, DNA damage, or gene expression pattern.
Conclusions These results demonstrated that thymidine can inhibit the TTFields-caused DNA damage and apoptosis, suggesting that combining TTFields and conventional treatments, such as chemotherapy, may enhance prognosis and decrease side efects compared with those of TTFields or conventional treatments alone.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Chen W / 2016 / Cancer stem cell quiescence and plasticity as major challenges in cancer therapy / Stem Cells Int
Dotiwala F / 2010 / Mad2prolongs DNA damage checkpoint arrest caused by a doublestrand break via a centromere-dependent mechanism / Curr Biol 20:328~332
Giladi M / 2015 / Mitotic spindle disruption by alternating electric felds leads to improper chromosome segregation and mitotic catastrophe in cancer cells / Sci Rep 5:18046
Gonzalez CF / 2005 / Harnessing dielectric forces for separations of cells, fne particles and macromolecules / J Chromatogr A 1079:59~68
Jeong H / 2014 / Inhibition of brain tumor cell proliferation by alternating electric felds / Appl Phys Lett 105:203703
Dotiwala F / 2010 / Mad2prolongs DNA damage checkpoint arrest caused by a doublestrand break via a centromere-dependent mechanism / Curr Biol 20:328~332
Giladi M / 2015 / Mitotic spindle disruption by alternating electric felds leads to improper chromosome segregation and mitotic catastrophe in cancer cells / Sci Rep 5:18046
Gonzalez CF / 2005 / Harnessing dielectric forces for separations of cells, fne particles and macromolecules / J Chromatogr A 1079:59~68
Jeong H / 2014 / Inhibition of brain tumor cell proliferation by alternating electric felds / Appl Phys Lett 105:203703
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