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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 351 - 359 (9page)
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The red sea bream iridovirus (RSIV) belonging to genus Megalocytivirus is responsible for red sea bream iridoviral disease (RSIVD) in marine and freshwater fishes. Although several diagnostic assays for RSIV have been developed, diagnostic sensitivity (DSe) and specificity (DSp) of real-time polymerase chain reaction (PCR) assays are not yet evaluated. In this study, we developed a TaqMan probe-based real-time PCR method and evaluated its DSe and DSp. To detect RSIV, the probe and primers were designed based on consensus sequences of the major capsid protein (MCP) genes from megalocytiviruses including RSIV, infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV). The probe and primers were shown to be specific for RSIV, ISKNV, and TRBIV-types megalocytiviruses. A 95% limit of detection (LOD95%) was determined to be 5.3 viral genome copies/μL of plasmid DNA containing the MCP gene from RSIV. The DSe and DSp of the developed real-time PCR assay for field samples (n = 112) were compared with those of conventional PCR assays and found to be 100% and 95.2%, respectively.
The quantitative results for SYBR Green and TaqMan probe-based real-time PCR were not significantly different. The TaqMan probe-based real-time PCR assay for RSIV may be used as an appropriate diagnostic tool for qualitative and quantitative analysis.
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참고문헌 (24)
참고문헌 신청
Arikawa E / 2008 / Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control(MAQC)study / BMC Genomics 9:328
Arya M / 2005 / Basic principles of real-time quantitative PCR / Expert Rev Mol Diagn 5:209~219
Chinchar VG / 2017 / ICTV virus taxonomy profile : Iridoviridae / J Gen Virol 98:890~891
Do JW / 2005 / Phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders Paralichthys olivaceus in Korea / Dis Aquat Organ 64:193~200
Inouye K / 1992 / Iridovirus infection of cultured red sea bream, Pagrus major / Fish Pathol 27:19~27
Arya M / 2005 / Basic principles of real-time quantitative PCR / Expert Rev Mol Diagn 5:209~219
Chinchar VG / 2017 / ICTV virus taxonomy profile : Iridoviridae / J Gen Virol 98:890~891
Do JW / 2005 / Phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders Paralichthys olivaceus in Korea / Dis Aquat Organ 64:193~200
Inouye K / 1992 / Iridovirus infection of cultured red sea bream, Pagrus major / Fish Pathol 27:19~27
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