지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
The CRISPR-Cas system is a microbial adaptive and heritable immune system, of which mechanism relies on the effectorribonucleoprotein (RNP) complex to degrade foreign genetic elements. The effector surveillance complex of the Type III-ACRISPR-Cas system has five Csm components, Csm1~Csm5. The Csm5 protein is placed in the crRNA 3' ends in theeffector RNP complex, leading to speculations that crRNA maturation may be catalyzed by the Csm5 subunit. However,the crystal structure and the detailed function of Csm5 still remain elusive. In this study, the Csm5 from Thermococcusonnurineus NA1 was purified and crystallized by the sitting drop method in the condition of 20% w/v PEG 8000 and 100mM CHES/sodium hydroxide pH 9.5 at 291K. The diffraction data were collected to a resolution of 3.5 A. The crystalbelonged to space group P43212, with unit cell parameters a=81.0 A, b=81.0 A, c=169.1 A. One protomer was presented inthe asymmetric unit with a corresponding VM of 2.10 A3 Da-1 and solvent content of 50%.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Cao, L / 2016 / Identification and functional study of type III-A CRISPR-Cas systems in clinical isolates of Staphylococcus aureus / Int J Med Microbiol 306:686~696
Deveau, H / 2010 / CRISPR/Cas system and its role in phage-bacteria interactions / Annu Rev Microbiol 64:475~493
Fagerlund, R. D / 2015 / The Cpf1 CRISPRCas protein expands genome-editing tools / Genome Biol 16:251
Garneau, J. E / 2010 / The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA / Nature 468:67~71
Han, W / 2017 / A type III-B CRISPRCas effector complex mediating massive target DNA destruction / Nucleic Acids Res 45:1983~1993
Deveau, H / 2010 / CRISPR/Cas system and its role in phage-bacteria interactions / Annu Rev Microbiol 64:475~493
Fagerlund, R. D / 2015 / The Cpf1 CRISPRCas protein expands genome-editing tools / Genome Biol 16:251
Garneau, J. E / 2010 / The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA / Nature 468:67~71
Han, W / 2017 / A type III-B CRISPRCas effector complex mediating massive target DNA destruction / Nucleic Acids Res 45:1983~1993
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