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학술저널
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유현진 (중앙대학교) 이운광 (중앙대학교) CUI WENYING (중앙대학교) Chung Wonseok (BioNano Health Guard Research Center) Shin Yong Beom (BioNano Health Guard Research Center) Kim Yeon-Sook (Chungnam National University School of Medicine) Baek Changyoon (Chung-Ang University) Min Junhong (Chung-Ang University)
저널정보
나노기술연구협의회 Nano Convergence Nano Convergence Vol.8 No.31
발행연도
2021.10
수록면
1 - 10 (10page)
DOI
10.1186/s40580-021-00281-8

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It is highly important to sensitively measure the abundance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on various surfaces. Here, we present a nucleic acid-based detection method consisting of a new sample preparation protocol that isolates only viruses, not the free RNA fragments already present on the surfaces of indoor human-inhabited environments, using a graphene oxide-coated microbead filter. Wet wipes (100 cm 2 ), not cotton swabs, were used to collect viruses from environmental surfaces with large areas, and viruses were concentrated and separated with a graphene oxide-coated microbead filter. Viral RNA from virus was recovered 88.10 ± 8.03% from the surface and free RNA fragment was removed by 99.75 ± 0.19% from the final eluted solution. When we tested the developed method under laboratory conditions, a 10-fold higher viral detection sensitivity (Detection limit: 1 pfu/100 cm 2 ) than the current commercial protocol was observed. Using our new sample preparation protocol, we also confirmed that the virus was effectively removed from surfaces after chemical disinfection; we were unable to measure the disinfection efficiency using the current commercial protocol because it cannot distinguish between viral RNA and free RNA fragments. Finally, we investigated the presence of SARS-CoV-2 and bacteria in 12 individual negative pressure wards in which patients with SARS-CoV-2 infection had been hospitalized. Bacteria (based on 16?S DNA) were found in all samples collected from patient rooms; however, SARS-CoV-2 was mainly detected in rooms shared by two patients.

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