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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.3
- 수록면
- 221 - 228 (8page)
- DOI
- 10.14348/molcells.2015.2170
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초록· 키워드
오류제보하기
Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa.
Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, p75NTR, S-100, and vimentin.
Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin notonly promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.
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참고문헌 (43)
참고문헌 신청
Armati, P. J. / 2014 / Clinical implications of Schwann cell biology / J. Peripher. Nerv. Syst 19:14~23
Berrocal, Y. A. / 2013 / Transplantation of Schwann cells in a collagen tube for the repair of large, segmental peripheral nerve defects in rats / J. Neurosurg 119:720~732
Betters, E. / 2010 / Analysis of early human neural crest development / Dev. Biol 344:578~592
Chen, P. / 2014 / The role of collagens in peripheral nerve myelination and function / Mol. Neurobiol 2014:1~10
Christopherson, G. T. / 2009 / The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation / Biomaterials 30:556~564
Berrocal, Y. A. / 2013 / Transplantation of Schwann cells in a collagen tube for the repair of large, segmental peripheral nerve defects in rats / J. Neurosurg 119:720~732
Betters, E. / 2010 / Analysis of early human neural crest development / Dev. Biol 344:578~592
Chen, P. / 2014 / The role of collagens in peripheral nerve myelination and function / Mol. Neurobiol 2014:1~10
Christopherson, G. T. / 2009 / The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation / Biomaterials 30:556~564
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