지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.10
- 수록면
- 855 - 869 (15page)
이용수
초록· 키워드
오류제보하기
BACKGROUND/OBJECTIVES: Atopic dermatitis (AD) is a chronic disease with an increasing incidence globally; therefore, there is a growing demand for natural compounds effective in treating dermatitis. In this study, the protective effects of Lycium barbarum leaves with and without chlorophyll (LLE and LLE[Ch-]) on AD were investigated in animal models of AD and HaCaT cells. Further, we investigated whether LLE and LLE(Ch-) show any differences in physiological activity.
MATERIALS/METHODS: AD was induced by 2,4-dinitrochlorobenzene (DNCB) for three weeks, while NC/Nga mice were fed LLE or LLE(Ch-) extracts for 7 weeks. Serum immunoglobulin E (IgE) and cytokine (tumor necrosis factor [TNF]-α, interleukin [IL]-6, and IL-4) concentrations and the degree of DNA fragmentation in lymphocytes were examined. A histopathological examination (haematoxylin & eosin staining and blue spots of toluidine) of the dorsal skin of mice was performed. To elucidate the mechanism of action, the expression of the thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) were measured in HaCaT cells.
RESULTS: Serum IgE and cytokines (TNF-α and IL-6) levels as well as DNA fragmentation of lymphocytes were significantly decreased in AD-induced mice treated with LLE or LLE(Ch-) compared to those of the control group. The epidermal thickness of the dorsal skin and mast cell infiltration in the LLE group significantly reduced compared to that in the control group. The LLE extracts showed no cytotoxicity up to 1,000 μg/mL in HaCaT cells. LLE or LLE(Ch-)-treated group showed a reduction of TARC and MDC in TNF-α-and IFN-γ-stimulated HaCaT cells.
CONCLUSIONS: These results suggest that LLE potentially improves inflammation by reducing the expression of chemokines that inhibit T helper 2 cell migration. LLE(Ch-) showed similar effects to LLE on blood levels of IgE, TNF-α and IL-6 and protein expression in HaCat cells, but the ultimate effect of skin improvement was not statistically significant. Therefore, both LLE and LLE(Ch-) can be used as functional materials to alleviate AD, but LLE(Ch-) appears to require more research to improve inflammation.
MATERIALS/METHODS: AD was induced by 2,4-dinitrochlorobenzene (DNCB) for three weeks, while NC/Nga mice were fed LLE or LLE(Ch-) extracts for 7 weeks. Serum immunoglobulin E (IgE) and cytokine (tumor necrosis factor [TNF]-α, interleukin [IL]-6, and IL-4) concentrations and the degree of DNA fragmentation in lymphocytes were examined. A histopathological examination (haematoxylin & eosin staining and blue spots of toluidine) of the dorsal skin of mice was performed. To elucidate the mechanism of action, the expression of the thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) were measured in HaCaT cells.
RESULTS: Serum IgE and cytokines (TNF-α and IL-6) levels as well as DNA fragmentation of lymphocytes were significantly decreased in AD-induced mice treated with LLE or LLE(Ch-) compared to those of the control group. The epidermal thickness of the dorsal skin and mast cell infiltration in the LLE group significantly reduced compared to that in the control group. The LLE extracts showed no cytotoxicity up to 1,000 μg/mL in HaCaT cells. LLE or LLE(Ch-)-treated group showed a reduction of TARC and MDC in TNF-α-and IFN-γ-stimulated HaCaT cells.
CONCLUSIONS: These results suggest that LLE potentially improves inflammation by reducing the expression of chemokines that inhibit T helper 2 cell migration. LLE(Ch-) showed similar effects to LLE on blood levels of IgE, TNF-α and IL-6 and protein expression in HaCat cells, but the ultimate effect of skin improvement was not statistically significant. Therefore, both LLE and LLE(Ch-) can be used as functional materials to alleviate AD, but LLE(Ch-) appears to require more research to improve inflammation.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Protective effect of Lycium barbarum leaf extracts on atopic dermatitis: in vitro and in vivo studies
Nutrition Research and Practice
2023 .10
-
Anti-inflammatory effect of Lycium Barbarum leaf on polarized human intestinal epithelial cells
한국생활과학회 학술대회논문집
2020 .11
-
Analysis of volatile compounds, betaine in goji berry (Lycium barbarum L.) powder prepared with various extraction and drying methods
한국분석과학회 학술대회
2022 .11
-
구기자와 맥문동 추출 혼합물의 항염증 효능
한국생활과학회 학술대회논문집
2024 .05
-
붉은 열매 구기자와 검은 열매 구기자의 이화학적 특성 비교 연구
한국식품조리과학회지
2017 .01
-
Inhibition of atopic dermatitis mice using a non-thermal argon plasma
한국진공학회 학술발표회초록집
2017 .02
-
Skin Microbiome Signatures in Atopic Dermatitis
한국미생물학회 학술대회논문집
2022 .10
-
Lycium Chinense의 열매 추출물과 잎 추출물의 항염 효능 연구
한국생활과학회 학술대회논문집
2018 .05
-
Oral Treatment with Probiotics Inhibits the Atopic Dermatitis in Mice
한국미생물학회 학술대회논문집
2017 .04
-
클로로필 제거 구기엽 추출물의 비알코올성 지방간 보호 효과
Journal of Nutrition and Health
2023 .04
-
Gut Microbiome Change Occurred by Diet Control in Atopic Dermatitis
한국미생물학회 학술대회논문집
2020 .10
-
Effects of Probiotics and Sodium Butyrate on Atopic Dermatitis
한국미생물학회 학술대회논문집
2017 .04
-
The Relationship between Gut Microbiota at 6 Months and Persistence of Atopic Dermatitis in Early Childhood
한국미생물학회 학술대회논문집
2019 .04
-
Differently Developed Infancy Gut Microbiome According to Severity of Atopic Dermatitis
한국미생물학회 학술대회논문집
2019 .04
-
Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells
Nutrition Research and Practice
2019 .04
-
Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells
Nutrition Research and Practice
2019 .04
-
Seeking for "The" Staphylococcus epidermidis Strain that Alleviates Atopic Dermatitis
한국미생물학회 학술대회논문집
2022 .10
-
Probiotic Mixture Regulates Immune Balance to Reduce Atopic Dermatitis Symptoms in Mice
한국미생물학회 학술대회논문집
2020 .10
-
성인의 아토피 피부염과 식생활평가지수와의 관계: 국민건강영양조사 제7기 (2016–2018년) 자료 이용
Journal of Nutrition and Health
2022 .10
-
Lycium chinense Miller 잎 에탄올 추출물의 항산화 및 항염 효능
한국생활과학회 학술대회논문집
2024 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-24-02-088080713