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논문 기본 정보

자료유형
학술저널
저자정보
Allison Suleiman D (Department of Food Science and Technology, Faculty of Agriculture and Agricultural Technology, Moddibo Adama University, Yola 640230, Nigeria) AdeelaYasid Nur (Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra, Malaysia, 43400 Serdang Selangor, Malaysia) Shariff Fairolniza Mohd (Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia) Abdul Rahman Nor’Aini (Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra, Malaysia, 43400 Serdang Selangor, Malaysia)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.2
발행연도
2024.2
수록면
436 - 456 (21page)
DOI
10.4014/jmb.2306.06050

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Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius SKF4 bacterium through polymerase chain reaction (PCR). The purified recombinant SpSKF4 protease was characterized, followed by evaluation of its possible industrial applications. The analysis of the gene sequence revealed an open reading frame (ORF) consisting of 1,206 bp, coding for a protein containing 401 amino acids. The cloned gene was expressed in Escherichia coli. The molecular weight of the enzyme was measured at 28 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The partially purified enzyme has its highest activity at a pH of 10 and a temperature of 80o C. In addition, the enzyme showed a half-life of 15 h at 80o C, and there was a 60% increase in its activity at 10 mM Ca2+ concentration. The activity of the protease was completely inhibited (100%) by phenylmethylsulfonyl fluoride (PMSF); however, the addition of sodium dodecyl sulfate (SDS) resulted in a 20% increase in activity. The enzyme was also stable in various organic solvents and in certain commercial detergents. Furthermore, the enzyme exhibited strong potential for industrial use, particularly as a detergent additive and for facilitating the recovery of silver from X-ray film.

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