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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 358 - 365 (8page)
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초록· 키워드
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An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60℃. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H2O2, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40℃). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.
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참고문헌 (52)
참고문헌 신청
Singh S / 2017 / Potential application spectrum of microbial proteases for clean and green industrial production / Energy Ecol. Env 2:370~386
Razzaq A / 2019 / Microbial proteases applications / Front. Bioeng. Biotechnol 7:110
Sarrouh B / 2012 / Up-To-Date insight on industrial enzymes applications and global market / J. Bioproces. Biotech 4:002
Niyonzima FN / 2014 / Detergent-compatible proteases : Microbial production, properties, and stain removal analysis / Prep. Biochem. Biotechnol 45:233~258
Rao MB / 1998 / Molecular and biotechnological aspects of microbial proteases / Microbiol. Mol. Biol. Rev 62:597~635
Razzaq A / 2019 / Microbial proteases applications / Front. Bioeng. Biotechnol 7:110
Sarrouh B / 2012 / Up-To-Date insight on industrial enzymes applications and global market / J. Bioproces. Biotech 4:002
Niyonzima FN / 2014 / Detergent-compatible proteases : Microbial production, properties, and stain removal analysis / Prep. Biochem. Biotechnol 45:233~258
Rao MB / 1998 / Molecular and biotechnological aspects of microbial proteases / Microbiol. Mol. Biol. Rev 62:597~635
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