지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 299 - 304 (6page)
이용수
초록· 키워드
오류제보하기
Liver transplantation is essential for patients with liver failure. Pig is known to be a most suitable species in xeno-transplantation of human organs. Hepatocytes are important sites in the body's metabolism and function. Glucose transporter in hepatocytes plays a role in regulation of blood glucose homeostasis. Epidermal growth factor (EGF) is known to be a regulator of physiological function in various cell systems. However, the effect of EGF on glucose activity in pig hepatocytes was not clearly elucidated. Thus, this study investigated the effect of EGF on glucose transporter and its related signal cascades in primary cultured pig hepatocytes. EGF inhibited glucose uptake in a dose (＞ 1 ng/㎖) dependent manner. EGF-induced decrease of [³H]-deoxyglucose uptake was blocked by AG 1478 (10-? M, an EGF receptor antagonist) genistein and herbymycin A (tyrosine kinase inhibitors, 10?? M), suggesting the role of activation and tyrosine phosphorylation of EGF receptor. In addition, EGF-induced decrease of [³H]-deoxyglucose uptake was prevented by mepacrine, AACOCF₃ (phospholipase A₂ inhibitors), indomethacin and ibuprofen (cyclooxygenase inhibitors). Indeed, EGF increased [³H]-arachidonic acid release and cyclooxygenase-2 (COX-2) expression. Moreover, EGF-induced stimulation of [³H]-arachidonic acid release was blocked by PD 98059 (a p44/42 mitogen activated protein kinase [MAPK] inhibitor) and SB 203580 (a p38 MAPK inhibitor). EGF-induced decrease of [³H]-deoxyglucose uptake was also prevented by these two MAPKs. However, EGF-induced stimulation of p44/42 MAPK and p38 MAPK was not blocked by mepacrine and indomethacin. In conclusion, EGF decreases glucose uptake via MAPK-COX-2 signal cascades in cultured pig hepatocytes.
목차
재료 및 방법
결과
고찰
감사의 글
참고문헌
참고문헌 (31)
참고문헌 신청
1. [학술지(정기간행물)] - Bogatcheva, N.V / 2003 / Mitogen-activated protein kinases in endothelial pathophysiology 51 : 341 ~ 352
2. [학술지(정기간행물)] - Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein-dye binding. Anal. Biochem. 72 : 248 ~ 256
3. [학술지(정기간행물)] - Chen, Z / 2002 / Morphology viability and functions of suckling pig hepatocytes cultured in serum-free medium at high density 19 : 184 ~ 191
4. [학술지(정기간행물)] - Diao, W.F / 2006 / Serum, liver, and kidney proteomic analysis for the alloxan-induced type I diabetic mice after insulin gene transfer of naked plasmid through electroporation 6 : 5837 ~ 5845
5. [학술지(정기간행물)] - Eisenberg, M.L / 2005 / Insulin receptor (IR) and glucose transporter 2 (GLUT2) proteins form a complex on the rat hepatocyte membrane 15 : 51 ~ 58
2. [학술지(정기간행물)] - Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein-dye binding. Anal. Biochem. 72 : 248 ~ 256
3. [학술지(정기간행물)] - Chen, Z / 2002 / Morphology viability and functions of suckling pig hepatocytes cultured in serum-free medium at high density 19 : 184 ~ 191
4. [학술지(정기간행물)] - Diao, W.F / 2006 / Serum, liver, and kidney proteomic analysis for the alloxan-induced type I diabetic mice after insulin gene transfer of naked plasmid through electroporation 6 : 5837 ~ 5845
5. [학술지(정기간행물)] - Eisenberg, M.L / 2005 / Insulin receptor (IR) and glucose transporter 2 (GLUT2) proteins form a complex on the rat hepatocyte membrane 15 : 51 ~ 58
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The Effect of Epidermal Growth Factor on Cell Proliferation and Its Related Signal Pathways in Pig Hepatocytes
대한의생명과학회지
2006 .09
-
Study on Epidermal Growth Factor (EGF) and Expression of EGF-Receptor (EGF-R) in Mouse IVF/IVC Embryo;I. Additive Effect of EGF and Expression of EGF-R on Mouse IVF Embryo Development
대한불임학회잡지
1997 .01
-
EGF, T3, HB-EGF 가 치주인대섬유모세포에 미치는 영향
대한소아치과학회지
2007 .01
-
Effects of Epidermal Growth Factor (EGF) and Anti-EGF on Early Embryonic Development in Mice
대한생식의학회 학술대회
1996 .01
-
Study on Epidermal Growth Factor (EGF) and Expression of EGF-Receptor (EGF-R) in Mouse IVF/IVC Embryo;II. Expression of EGF-R on the Inner Cell Mass (ICM) of Mouse IVF/IVC Blastocyst
대한불임학회잡지
1997 .01
-
Epidermal Growth Factor(EGF)가 생쥐 초기배아의 발생에 미치는 영향
대한불임학회잡지
1995 .01
-
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats
대한물리치료사학회지
2006 .01
-
피부투과 기능성 펩타이드를 이용한 경피투과성 상피세포성장인자의 개발
대한화장품학회지
2019 .01
-
Multiple Roles of Phospholipase D in Growth Factor Signaling
대한약학회 학술대회
2003 .01
-
Controlled Release of Epidermal Growth Factor (EGF) from EGF-loaded Polymeric Nanoparticles Composed of Polystyrene as Core and Poly(methacrylic acid) as Corona in vitro
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2003 .01
-
In Vivo Development of Mouse IVF/IVC Embryo Treated with Epidermal Growth Factor (EGF)
대한불임학회잡지
1997 .01
-
상피세포성장인자를 함유한 동결건조-재분산 리포좀의 입도분포 및 봉입률
약학회지
1996 .01
-
Effects of High Glucose on Cell Proliferation in Cultured Suckling Pig Hepatocytes and its Related Signal Pathways
Laboratory Animal Research
2006 .09
-
발생중 마우스 배자에서 Transforming Growth Factor-α와 Epidermal Growth Factor의 발현차이
해부·생물인류학
1995 .06
-
Effects of Epidermal Growth Factor (EGF) on Mouse IVF Embryo Development and Their Cell Number
대한생식의학회 학술대회
1996 .01
-
The role of smad7 on epidermal growth factor (EGF) signaling pathway
대한외과학회 학술대회 초록집
2009 .11
-
양극성 장애에서 표피성장인자의 유전적 다형성에 대한 연합연구
신경정신의학
2007 .01
-
Effect of Epidermal Growth Factor on In Vitro Maturation in Pig Immature Oocytes;II. Effect of Epidermal Growth Factor on GVBD
대한불임학회잡지
1996 .01
-
Tumor Growth Suppression and Enhanced Radioresponse by an Exogenous Epidermal Growth Factor in Mouse Xenograft Models with A431 Cells
Cancer Research and Treatment
2015 .01
-
Heparin Binding Epidermal Growth Factor가 인간 방광근육의 비대 / 비후에 미치는 영향
International Neurourology Journal
2008 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-510-016364276