지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 10 - 18 (9page)
이용수
초록· 키워드
오류제보하기
Purpose: Many researchers have tried to develop animal models that mimic the human immune system, e.g. a humanized mouse model, to improve the engraftment of hematopoietic stem cells and develop human immune cells in an animal model. This study evaluated the feasibility of the cultured human umbilical cord blood (hUCB)-derived CD34? cells for cell expansion, in Rag2<SUP>-/-</SUP>γc<SUP>-/-</SUP> mice, and establish co-transplantation with human fetal thymus/liver tissue (Thy/Liv) under the kidney capsule.
Methods: Co-transplantation of hUCB-derived CD34? cells with Thy/Liv was performed. The hUCB-derived CD34? cells were prepared by freshly thawing (G1) and culturing for 7 days with two types of cytokine combinations (G2, G3). The CD45? cell populations were measured at 6, 8, 10 and 16 weeks in the peripheral blood. The splenocytes were cultured with mitogenic stimuli (PHA -L or IL-2) at 20 weeks posttransplantation, and the proliferation of human immune cells was evaluated.
Results: There were no significant differences in the human CD45? cell populations at 6, 8, 10 and 16 weeks post-transplantation between the groups. In the cultured splenocytes at 20 weeks post-transplant with PHA-L or IL-2, there was remarkable expansion of CD3? cells in the three groups. Although no CD19? cells were detected in the spleen, human Ig G was detected in the sera of these mice.
Conclusion: The cultured and expanded hUCB-derived cells with cytokine combinations might be a feasible cell source in humanized mouse modeling. In addition, human immune cells can be reconstituted from the co-transplantation of Thy/Liv and cultured hUCB-derived CD34? cells.
Methods: Co-transplantation of hUCB-derived CD34? cells with Thy/Liv was performed. The hUCB-derived CD34? cells were prepared by freshly thawing (G1) and culturing for 7 days with two types of cytokine combinations (G2, G3). The CD45? cell populations were measured at 6, 8, 10 and 16 weeks in the peripheral blood. The splenocytes were cultured with mitogenic stimuli (PHA -L or IL-2) at 20 weeks posttransplantation, and the proliferation of human immune cells was evaluated.
Results: There were no significant differences in the human CD45? cell populations at 6, 8, 10 and 16 weeks post-transplantation between the groups. In the cultured splenocytes at 20 weeks post-transplant with PHA-L or IL-2, there was remarkable expansion of CD3? cells in the three groups. Although no CD19? cells were detected in the spleen, human Ig G was detected in the sera of these mice.
Conclusion: The cultured and expanded hUCB-derived cells with cytokine combinations might be a feasible cell source in humanized mouse modeling. In addition, human immune cells can be reconstituted from the co-transplantation of Thy/Liv and cultured hUCB-derived CD34? cells.
목차
서론
방법
결과
고찰
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
결장암에 대한 활성 자연살해세포의 항암효능
약학회지
2010 .01
-
CD40L 발현 K562 세포주를 이용한 시험관내 B 세포 증식과 항원제공능 증가
Immune Network
2007 .06
-
Role for CD40 and CD40L Expression in Generating CD8 T Cell Response to Minor Histcompatibility Antigen, H60
Immune Network
2007 .12
-
Regulation of Inflammation by Bidirectional Signaling through CD137 and Its Ligand
Immune Network
2012 .10
-
Expression and Function of TLR2 on CD4 Versus CD8 T Cells
Immune Network
2009 .08
-
Effect of IL-4 on the Development and Function of Memory-like CD8 T Cells in the Peripheral Lymphoid Tissues
Immune Network
2016 .04
-
Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60
Immune Network
2012 .06
-
비소세포성폐암에 대한 자연살해세포의 항암효능
약학회지
2011 .01
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
CD43 Expression Regulated by IL-12 Signaling Is Associated with Survival of CD8 T Cells
Immune Network
2010 .10
-
Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study
Journal of Clinical Neurology
2017 .01
-
시호(柴胡)가 MC 및 Mouse의 염증관련 cytokine 분비에 미치는 영향
동의생리병리학회지
2009 .01
-
A Therapeutic Effect of Pinellia Ternata via the Increase of CD4+CD25+ Regulatory T Cells and the Suppression of CD3+CCR3+ Cellular Infiltration During Allergic Airway Inflammation
대한본초학회지(본초분과학회지)
2009 .01
-
The effects of HLA mismatched cells in increasing the frequency of CD4+CD8+ thymocytes in humanized mice model
한국실험동물학회 학술발표대회 논문집
2019 .07
-
CD29 및 CD98 활성 매개에 의한 Jurkat T 세포의 유착과 그 활용
약학회지
2009 .01
-
CD137-CD137 Ligand Interactions in Inflammation
Immune Network
2009 .06
-
조절 T세포에 미치는 梔子의 효과
대한한방내과학회지
2010 .01
-
Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions
Immune Network
2003 .09
-
마행감석탕가감방(麻杏甘石湯加減方)이 천식모델 생쥐의 CD3, CD4, CD8 세포에 미치는 영향
논문집 : 대전대학교 한의학연구소. 한의학편
2008 .01
-
Cross-linking of CD80 and CD86 Diminishes Expression of CD54 on EBV-transformed B Cells through Inactivation of RhoA and Ras
Immune Network
2011 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0