지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 25 - 33 (9page)
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초록· 키워드
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Purpose: The detection of papillary thyroid carcinoma (PTC) is increasing these days; however, there is currently no satisfactory definitive preoperative diagnostic method. Fine-needle aspiration cytology (FNAC) is now the most accurate method to diagnose PTC preoperatively. It is known that the specificity of BRAF mutation is high in papillary thyroid carcinoma. Therefore, detection of BRAF mutation using a FNAC slide can be helpful to diagnose PTC preoperatively.
Methods: 13 patients with benign disease, 36 patients with PTC and 23 patients with an indeterminate diagnosis as determined histologically on the FNAC slide were evaluated to detect BRAF mutation with using FNAC slides and intraoperative fresh tissue.
Results: Mutation was detected using direct sequencing and the colorimetric method. The frequency of BRAF mutation was 86.3% for all the PTC cases. The concordance between the colorimetric method and direct sequencing was 57.1%. During DNA extraction from the FNAC slide, the DNA damage is so severe that direct sequencing is succeeded in only one case.
Conclusion: We have to take measures to overcome and prevention DNA damage during extraction. The colorimetric method is not reliable.
Methods: 13 patients with benign disease, 36 patients with PTC and 23 patients with an indeterminate diagnosis as determined histologically on the FNAC slide were evaluated to detect BRAF mutation with using FNAC slides and intraoperative fresh tissue.
Results: Mutation was detected using direct sequencing and the colorimetric method. The frequency of BRAF mutation was 86.3% for all the PTC cases. The concordance between the colorimetric method and direct sequencing was 57.1%. During DNA extraction from the FNAC slide, the DNA damage is so severe that direct sequencing is succeeded in only one case.
Conclusion: We have to take measures to overcome and prevention DNA damage during extraction. The colorimetric method is not reliable.
목차
서론
방법
결과
고찰
결론
REFERENCES
참고문헌 (30)
참고문헌 신청
Nguyen GK , 2005 , Fine-needle aspiration of the thyroid: an overview , Cytojournal 2 : 12 ~
Hamburger JI , 1986 , Fine needle biopsy of thyroid nodules: avoiding the pitfalls , N Y State J Med 86 : 241 ~ 249
Schmitt FC , 2006 , Thyroid cytology: FNA is still the best diagnostic approach , Cytopathol 17 : 211 ~ 212
Boyd LA , 1998 , Preoperative evaluation and predictive value of fine-needle aspiration and frozen section of thyroid nodules , J Am Coll Surg 187 : 494 ~ 502
Wong Z , 2004 , Role of intraoperative frozen section in the management of thyroid nodules , ANZ J Surg 74 : 1052 ~ 1055
Hamburger JI , 1986 , Fine needle biopsy of thyroid nodules: avoiding the pitfalls , N Y State J Med 86 : 241 ~ 249
Schmitt FC , 2006 , Thyroid cytology: FNA is still the best diagnostic approach , Cytopathol 17 : 211 ~ 212
Boyd LA , 1998 , Preoperative evaluation and predictive value of fine-needle aspiration and frozen section of thyroid nodules , J Am Coll Surg 187 : 494 ~ 502
Wong Z , 2004 , Role of intraoperative frozen section in the management of thyroid nodules , ANZ J Surg 74 : 1052 ~ 1055
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UCI(KEPA) : I410-ECN-0101-2013-514-002693732