Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L.

Hossein Mahmoudvand; Fatemeh Ezzatkhah; Fariba Sharififar; Iraj Sharifi; Ebrahim Saedi Dezaki

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자료유형: 학술저널

저자정보: Hossein Mahmoudvand (Kerman University of Medical Sciences) Fatemeh Ezzatkhah (Kerman University of Medical Sciences) Fariba Sharififar (Kerman University of Medical Sciences) Iraj Sharifi (Kerman University of Medical Sciences) Ebrahim Saedi Dezaki (Kerman University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.1

발행연도: 2015.2

수록면: 21 - 27 (7page)

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Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC<SUB>50</SUB> values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC<SUB>50</SUB> values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

#Leishmania tropica #cutaneous leishmaniasis #myrtle #promastigote #amastigote #J774-A1 cell

참고문헌 (28)

참고문헌 신청

World Health Organization / 2010 / Control of the leishmaniases :5~12

Desjeux P / 2004 / Leishmaniasis: current situation and new perspectives / Comp Immunol Microbiol Infect Dis 27:305~318

Mahmoudvand H / 2014 / In vitro inhibitory effect of Berberis vulgaris (Berberidaceae) and its main component, berberine against different Leishmania species / Iranian J Parasitol 9:28~36

Santos DO / 2008 / Leishmaniasis treatment-a challenge that remains: a review / Parasitol Res 103:1~10

Rocha LG / 2005 / A review of natural products with antileishmanial activity / Phytomedicine 12:514~535

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