Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Guochao Zheng; Wei Hu; Yuanjia Liu; Qin Luo; Liping Tan; Guoqing Li

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자료유형: 학술저널

저자정보: Guochao Zheng (South China Agricultural University) Wei Hu (South China Agricultural University) Yuanjia Liu (South China Agricultural University) Qin Luo (South China Agricultural University) Liping Tan (South China Agricultural University) Guoqing Li (South China Agricultural University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.1

발행연도: 2015.2

수록면: 119 - 124 (6page)

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The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

#Giardia duodenalis #multilocus genotyping #zoonotic assemblage #cat #China

참고문헌 (26)

참고문헌 신청

Thompson RC / 2000 / Nomenclature and genetic groupings of Giardia infecting mammals / Parasitol Today 16:210~213

Monis PT / 2009 / Variation in Giardia: towards a taxonomic revision of the genus / Trends Parasitol 25:93~100

Feng Y / 2011 / Zoonotic potential and molecular epidemiology of Giardia species and giardiasis / Clin Microbiol Rev 24:110~140

Yong TS / 2000 / Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences / J Parasitol 86:887~891

Wang R / 2011 / Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China / Exp Parasitol 127:42~45

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