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논문 기본 정보

자료유형
학술저널
저자정보
Chung Eun Hwang (Gyeongnam National University of Science and Technology) Dong Hoon Lee (Gyeongsang National University) Baolo Kim (Gyeongnam National University of Science and Technology) Ok Soo Joo (Gyeongnam National University of Science and Technology) Su Cheol Kim (Gyeongnam National University of Science and Technology) Jin Hwan Lee (Ministry of Environment) Su Young Hong (Kolmar BNH) A Ra Choi (Gyeongnam National University of Science and Technology) Kye Man Cho (Gyeongnam National University of Science and Technology)
저널정보
한국식품저장유통학회 Food Science and Preservation 한국식품저장유통학회지 제25권 제4호
발행연도
2018.7
수록면
461 - 470 (10page)
DOI
10.11002/kjfp.2018.25.4.461

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This study aimed to produce fermented soy-powder milk (FSPM) with Lactobacillus plantarum P1201 and to evaluate its anti-obesity activity. Isoflavone and conjugated linoleic acid (CLA) of unfermented soy-powder milk (UFSPM) and FSPM and were analyzed via high-pressure liquid chromatography (HPLC) and gas chromatography (GC). Their inhibitory activities against α-glucosidase, α-amylase, and pancreatic lipase were assayed. Their anti-obesity activities were evaluated on the basis of their inhibitory effects on adipocyte differentiation in 3T3-L1 cells, and the expression of mRNAs associated with adipogenesis and lipid metabolism were analyzed via real time-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR). FSPM with L. plantarum P1201 increased the isoflavone aglycones (daidzein, glycitein, and genistein) content and produced CLA in soy-powder milk (SPM), both of which possessed bio-activity. Both UFSPM and FSPM showed dose-dependent inhibitory activity for α-glucosidase, α-amylase, and pancreatic lipase. FSPM, but not UFSPM, suppressed adipogenesis in 3T3-L1 cells and reduced their triglyceride content by 23.1% after treatment with 1,000 μg/mL of FSPM, compared with the control group. The anti-obesity effect of FSPM can be attributed to CLA and isoflavone aglycones, which targeted CCAAT/enhancer binding protein α (C/EBP-α) and down-regulated lipoprotein lipase (LPL), adiponectin, adipocyte fatty acid-binding protein (aP2), fatty acid synthase (FAS), and acetyl CoA carboxylase (ACC) mRNA. Furthermore, FSPM enhanced the inhibitory activity of glucosidase and pancreatic enzymes and anti-obesity activity. Further studies are required to investigate whether the anti-obesity effect of FSPM persists in an in vivo mouse model of diet-induced obesity.

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Abstract
Introduction
Methods and materials
Results and discussion
Conclusion
References

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