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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 116 - 124 (9page)
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초록· 키워드
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Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes.
Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes.
Results: E. coli engineered with GT95<SUP>syn</SUP> selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing p2GT95<SUP>syn</SUP>K1, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth.
Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.
Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes.
Results: E. coli engineered with GT95<SUP>syn</SUP> selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing p2GT95<SUP>syn</SUP>K1, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth.
Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (32)
참고문헌 신청
Nicol RW / 2011 / Ginsenosides as host resistance factors in American ginseng(Panax quinquefolius) / Can J Bot 80:557~562
Cho WCS / 2006 / Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats / Eur J Pharmacol 550:173~179
Wang T / 2016 / Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: a review / J Ethnopharmacol 188:234~258
Yang WZ / 2014 / Saponins in the genus Panax L. (Araliaceae): a systematic review of their chemical diversity / Phytochemistry 106:7~24
Gao B / 2014 / Platelet P2Y12 receptor involved in the hemostatic effect of notoginsenoside Ft1, a saponin isolated from Panax notoginseng / Br J Pharmacol 171:214~223
Cho WCS / 2006 / Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats / Eur J Pharmacol 550:173~179
Wang T / 2016 / Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: a review / J Ethnopharmacol 188:234~258
Yang WZ / 2014 / Saponins in the genus Panax L. (Araliaceae): a systematic review of their chemical diversity / Phytochemistry 106:7~24
Gao B / 2014 / Platelet P2Y12 receptor involved in the hemostatic effect of notoginsenoside Ft1, a saponin isolated from Panax notoginseng / Br J Pharmacol 171:214~223
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UCI(KEPA) : I410-ECN-0101-2019-524-000425212