지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
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The melanose disease causing Diaporthe citri is important pathogen in citrus plants worldwide. Currently, there are few researches related to biological control of melanose disease. In this study, we investigated control of Diaporthe citri by Bacillus amyloliquefaciens Y1 isolated from the soil. Low-cost fertilizer based PB medium and large-scale fermenter have been developed for field application of biological control. We prove that Y1 can be successfully grown in large-scale fermenters using PB medium without contamination. In addition, Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1), present better growth inhibition of D. citri than small-scale cultivation in TSB medium (ST-Y1). Microscopic observations also showed that pathogen mycelial morphology was significantly affected by BCF, presumably due to metabolites produced by Y1. Finally, in pot experiment, pre-treatment with fungicide and Y1 to leaves significantly reduced disease incidence compared to the control group. This study showed that Y1 incubated with fertilizer based medium inhibited the occurrence of D. citri and could be grown in large scale field conditions without contamination as well as cost optimization.
목차
ABSTRACT
Introduction
Materials and Methods
Results and Discussion
Conclusions
References
참고문헌 (22)
참고문헌 신청
Ahmad, F. 2011. Rhizosphere and root colonization by bacterial inoculants and their monitoring methods: a critical area in PGPR research, p. 363-391. In: Ahmad et al.(ed.) Microbes and Microbial technology. Springer, New York, USA.
Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20(8):338-343.
Glick, B. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41:109-117.
Huang, F., X. Hou, M.M. Dewdney, Y. Fu, G. Chen, K.D. Hyde, and H. Li, 2013. Diaporthe species occurring on citrus in China. Fungal Divers. 61(1):237-250.
Hyun, J.W., J.H. Park, K.S. Kim, G.J. Park, and S.H. Yoon, 2011. College tree, tangerine story. RDA. Suwon, Korea.
Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20(8):338-343.
Glick, B. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41:109-117.
Huang, F., X. Hou, M.M. Dewdney, Y. Fu, G. Chen, K.D. Hyde, and H. Li, 2013. Diaporthe species occurring on citrus in China. Fungal Divers. 61(1):237-250.
Hyun, J.W., J.H. Park, K.S. Kim, G.J. Park, and S.H. Yoon, 2011. College tree, tangerine story. RDA. Suwon, Korea.
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UCI(KEPA) : I410-ECN-0101-2019-523-000765423