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논문 기본 정보

자료유형
학술저널
저자정보
Dong-Ryul Lee (Chonnam National University) Chaw Ei Htwe Maung (Chonnam National University) Ajuna Henry (Chonnam National University) Kil-Yong Kim (Chonnam National University)
저널정보
한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제52권 제2호
발행연도
2019.5
수록면
84 - 92 (9page)

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The melanose disease causing Diaporthe citri is important pathogen in citrus plants worldwide. Currently, there are few researches related to biological control of melanose disease. In this study, we investigated control of Diaporthe citri by Bacillus amyloliquefaciens Y1 isolated from the soil. Low-cost fertilizer based PB medium and large-scale fermenter have been developed for field application of biological control. We prove that Y1 can be successfully grown in large-scale fermenters using PB medium without contamination. In addition, Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1), present better growth inhibition of D. citri than small-scale cultivation in TSB medium (ST-Y1). Microscopic observations also showed that pathogen mycelial morphology was significantly affected by BCF, presumably due to metabolites produced by Y1. Finally, in pot experiment, pre-treatment with fungicide and Y1 to leaves significantly reduced disease incidence compared to the control group. This study showed that Y1 incubated with fertilizer based medium inhibited the occurrence of D. citri and could be grown in large scale field conditions without contamination as well as cost optimization.

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ABSTRACT
Introduction
Materials and Methods
Results and Discussion
Conclusions
References

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UCI(KEPA) : I410-ECN-0101-2019-523-000765423