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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.6
- 수록면
- 488 - 495 (8page)
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초록· 키워드
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Background: Timosaponin AIII (TA3) is a steroidal saponin extracted from Anemarrhena asphodeloides. Here, we investigated the anticancer effects of TA3 in MG63 human osteosarcoma cells. TA3 attenuates migration and invasion of MG63 cells via regulations of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, which are involved with cancer metastasis in various cancer cells. TA3 reduced enzymatic activities and transcriptional expressions of MMP-2 and MMP-9 in MG63 cells. TA3 also inhibited Src, focal adhesion kinase, extracellular signal-regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, b-catenin, and cAMP response element binding signaling, which regulate migration and invasion of cells. TA3 induced apoptosis of MG63 cells via regulations of caspase-3, caspase-7, and poly(ADP-ribose) polymerase (PARP). Then, we tested several ginsenosides to be used in combination with TA3 for the synergistic anticancer effects. We found that ginsenosides Rb1 and Rc have synergistic effects on TA3-induced apoptosis in MG63 cells.
Methods: We investigated the anticancer effects of TA3 and synergistic effects of various ginseng saponins on TA3-induced apoptosis in MG63 cells. To test antimetastatic effects, we performed wound healing migration assay, Boyden chamber invasion assays, gelatin zymography assay, and Western blot analysis. Annexin V/PI staining apoptosis assay was performed to determine the apoptotic effect of TA3 and ginsenosides.
Results: TA3 attenuated migration and invasion of MG63 cells and induced apoptosis of MG63 cells. Ginsenosides Rb1 and Rc showed the synergistic effects on TA3-induced apoptosis in MG63 cells.
Conclusions: The results strongly suggest that the combination of TA3 and the two ginsenosides Rb1 and Rc may be a strong candidate for the effective antiosteosarcoma agent.
Methods: We investigated the anticancer effects of TA3 and synergistic effects of various ginseng saponins on TA3-induced apoptosis in MG63 cells. To test antimetastatic effects, we performed wound healing migration assay, Boyden chamber invasion assays, gelatin zymography assay, and Western blot analysis. Annexin V/PI staining apoptosis assay was performed to determine the apoptotic effect of TA3 and ginsenosides.
Results: TA3 attenuated migration and invasion of MG63 cells and induced apoptosis of MG63 cells. Ginsenosides Rb1 and Rc showed the synergistic effects on TA3-induced apoptosis in MG63 cells.
Conclusions: The results strongly suggest that the combination of TA3 and the two ginsenosides Rb1 and Rc may be a strong candidate for the effective antiosteosarcoma agent.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (36)
참고문헌 신청
Broadhead ML / 2011 / The molecular pathogenesis of osteosarcoma: a review / Sarcoma 2011
Ando K / 2013 / Current therapeutic strategies and novel approaches in osteosarcoma / Cancers 5(2):591~616
Takeda Y / 2001 / Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge / J Gastroenterol 36(2):79~90
Fu YL / 2008 / Pennogenin glycosides with a spirostanol structure are strong platelet agonists : structural requirement for activity and mode of platelet agonist synergism / J Thromb Haemostasis 6(3):524~533
Yang B-Y / 2016 / Steroidal saponins from the rhizomes of Anemarrhena asphodeloides / Molecules 21(8):1075
Ando K / 2013 / Current therapeutic strategies and novel approaches in osteosarcoma / Cancers 5(2):591~616
Takeda Y / 2001 / Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge / J Gastroenterol 36(2):79~90
Fu YL / 2008 / Pennogenin glycosides with a spirostanol structure are strong platelet agonists : structural requirement for activity and mode of platelet agonist synergism / J Thromb Haemostasis 6(3):524~533
Yang B-Y / 2016 / Steroidal saponins from the rhizomes of Anemarrhena asphodeloides / Molecules 21(8):1075
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