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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 62 - 68 (7page)
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초록· 키워드
오류제보하기
Background: Acinetobacter species are the leading cause of bloodstream infection (BSI), but their correct identification is challenging. We evaluated the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based VITEK MS (bioMérieux, France), and two automated systems, VITEK 2 (bioMérieux) and MicroScan (Siemens, USA) for identification of Acinetobacter BSI isolates.
Methods: A total of 187 BSI isolates recovered at a university hospital in Korea between 2010 and 2012 were analyzed. The identification results obtained using VITEK MS and two automated systems were compared with those of rpoB sequencing.
Results: Of 187 isolates analyzed, 176 were identified to the species level by rpoB sequencing: the Acinetobacter baumannii group (ABG; 101 A. baumannii, 43 A. nosocomialis, 10 A. pittii isolates) was most commonly identified (82.4%), followed by Acinetobacter genomic species 13BJ/14TU (5.3%), A. ursingii (2.1%), A. soli (2.1%), A. bereziniae (1.1%), and A. junii (1.1%). Correct identification rates to the species group (ABG) level or the species level was comparable among the three systems (VITEK MS, 90.3%; VITEK 2, 89.2%; MicroScan, 86.9%). However, VITEK MS generated fewer misidentifications (0.6%) than VITEK 2 (10.8%) and MicroScan (13.1%) (P<0.001). In addition, VITEK MS demonstrated higher specificity (100%) for discrimination between ABG and non-ABG isolates than the other systems (both, 31.8%) (P<0.001).
Conclusions: The VITEK MS system is superior to the VITEK 2 and MicroScan systems for identification of Acinetobacter BSI isolates, with fewer misidentifications and better discrimination between the ABG and non-ABG isolates.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Bernards AT / 1996 / Evaluation of the ability of a commercial system to identify Acinetobacter genomic species / Eur J Clin Microbiol Infect Dis 15 : 303 ~ 308
Chuang YC / 2011 / Influence of genospecies of Acinetobacter baumannii complex on clinical outcomes of patients with acinetobacter bacteremia / Clin Infect Dis 52 : 352 ~ 360
Dijkshoorn L / 2007 / An increasing threat in hospitals : multidrug-resistant Acinetobacter baumannii / Nat Rev Microbiol 5 : 939 ~ 951
Dubois D / 2012 / Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in ro
Gundi VA / 2009 / Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species / Microbiology 155 : 23
Chuang YC / 2011 / Influence of genospecies of Acinetobacter baumannii complex on clinical outcomes of patients with acinetobacter bacteremia / Clin Infect Dis 52 : 352 ~ 360
Dijkshoorn L / 2007 / An increasing threat in hospitals : multidrug-resistant Acinetobacter baumannii / Nat Rev Microbiol 5 : 939 ~ 951
Dubois D / 2012 / Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in ro
Gundi VA / 2009 / Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species / Microbiology 155 : 23
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