지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 530 - 536 (7page)
이용수
초록· 키워드
오류제보하기
Background: Next-generation sequencing is increasingly used for taxonomic identification of pathogenic bacterial isolates. We evaluated the performance of a newly introduced whole genome-based bacterial identification system, TrueBac ID (ChunLab Inc., Seoul, Korea), using clinical isolates that were not identified by three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems and 16S rRNA gene sequencing. Methods: Thirty-six bacterial isolates were selected from a university-affiliated hospital and a commercial clinical laboratory. Species was identified by three MALDI-TOF MS systems: Bruker Biotyper MS (Bruker Daltonics, Billerica, MA, USA), VITEK MS (bioMérieux, Marcy l’Étoile, France), and ASTA MicroIDSys (ASTA Inc., Suwon, Korea). Whole genome sequencing was conducted using the Illumina MiSeq system (Illumina, San Diego, CA, USA), and genome-based identification was performed using the TrueBac ID cloud system (www.truebacid.com). Results: TrueBac ID assigned 94% (34/36) of the isolates to known (N=25) or novel (N=4) species, genomospecies (N=3), or species group (N=2). The remaining two were identified at the genus level. Conclusions: TrueBac ID successfully identified the majority of isolates that MALDI-TOF MS failed to identify. Genome-based identification can be a useful tool in clinical laboratories, with its superior accuracy and database-driven operations.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Vouga M / 2016 / Emerging bacterial pathogens : the past and beyond / Clin Microbiol Infect 22:12~21
Croxatto A / 2012 / Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology / FEMS Microbiol Rev 36:380~407
Van den Abeele AM / 2018 / Filling the gaps in clinical proteomics : a do-it-yourself guide for the identification of the emerging pathogen Arcobacter by matrix-assisted laser desorption ionization-time of flight mass spectrometry / J Microbiol Methods 152:92~97
Chun J / 2014 / Integrating genomics into the taxonomy and systematics of the Bacteria and Archaea / Int J Syst Evol Microbiol 64:316~324
Yoon SH / 2017 / Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies / Int J Syst Evol Microbiol 67:1613~1617
Croxatto A / 2012 / Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology / FEMS Microbiol Rev 36:380~407
Van den Abeele AM / 2018 / Filling the gaps in clinical proteomics : a do-it-yourself guide for the identification of the emerging pathogen Arcobacter by matrix-assisted laser desorption ionization-time of flight mass spectrometry / J Microbiol Methods 152:92~97
Chun J / 2014 / Integrating genomics into the taxonomy and systematics of the Bacteria and Archaea / Int J Syst Evol Microbiol 64:316~324
Yoon SH / 2017 / Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies / Int J Syst Evol Microbiol 67:1613~1617
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
새로운 말디토프 질량 분석 장비인 ASTA MicroIDSys의 임상 균주를 이용한 균종 동정 성능 평가
Annals of Clinical Microbiology
2020 .01
-
Applications of MALDI-TOF Mass Spectrometry in Clinical Microbiology
대한의생명과학회지
2022 .09
-
A Novel Species of the Genus Arsenicicoccus Isolated From Human Blood Using Whole-Genome Sequencing
Annals of Laboratory Medicine
2021 .01
-
Performance of the ASTA MicroIDSys, a Novel MALDI-TOF MS Platform for Identifying Various Acinetobacter Species: Comparison with the Bruker Biotyper
Laboratory Medicine Online
2022 .07
-
MALDI-TOF 질량분석기를 이용한 바실러스균의 동정
Annals of Clinical Microbiology
2016 .01
-
Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Clinical Microbiology: What Are the Current Issues?
Annals of Laboratory Medicine
2017 .01
-
Comparison of a New Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform, ASTA MicroIDSys, With Bruker Biotyper for Species Identification
Annals of Laboratory Medicine
2017 .01
-
MALDI-TOF MS의 임상검사실 내 적용과 미생물검사의 패러다임 변화
Laboratory Medicine Online
2015 .01
-
Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts
Annals of Laboratory Medicine
2018 .01
-
Identification of Acinetobacter Species Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Annals of Laboratory Medicine
2016 .01
-
Comparison of MALDI-TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates
Yonsei Medical Journal
2015 .01
-
Identification of Salmonella spp. from porcine salmonellosis by matrix-assisted laser desorption ionization-time of flight mass spectrometry
한국가축위생학회지
2018 .01
-
Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria
Annals of Laboratory Medicine
2015 .01
-
Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System
Annals of Laboratory Medicine
2016 .01
-
Evaluation of Two Commercial Kits for Rapid Pathogen Identification Directly From Positive Blood Cultures by Matrix-Associated Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Annals of Clinical Microbiology
2023 .03
-
Performance Comparison Between Fourier-Transform Infrared Spectroscopy–based IR Biotyper and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Strain Diversity
Annals of Laboratory Medicine
2023 .03
-
Utility of Conventional Culture and MALDI-TOF MS for Identification of Microbial Communities in Bronchoalveolar Lavage Fluid in Comparison with the GS Junior Next Generation Sequencing System
Annals of Laboratory Medicine
2018 .01
-
Substantial Improvement in Nontuberculous Mycobacterial Identification Using ASTA MicroIDSys Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with an Upgraded Database
Annals of Laboratory Medicine
2022 .05
-
Performance of Matrix-Assisted Laser Desorption Ionization Time-of-Fight Mass Spectrometry for Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus (MRSA): First Report of a Relation Between Protein Peaks and MRSA spa Type
Annals of Laboratory Medicine
2017 .01
-
Direct Identification of Urinary Tract Pathogens From Urine Samples Using the Vitek MS System Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Annals of Laboratory Medicine
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0