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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 325 - 334 (10page)
이용수
초록· 키워드
오류제보하기
Background: Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has recently become the method of choice for identifying bacterial species. The purpose of this study was to evaluate the ability of MALDI-TOF MS (Bruker Daltonics GmbH, Germany) in combination with an improved database to identify various Acinetobacter species.
Methods: A total of 729 Acinetobacter clinical isolates were investigated, including 447 A. baumannii, 146 A. nosocomialis, 78 A. pittii, 18 A. ursingii, 9 A. bereziniae, 9 A. soli, 4 A. johnsonii, 4 A. radioresistens, 3 A. gyllenbergii, 3 A. haemolyticus, 2 A. lwoffii, 2 A. junii, 2 A. venetianus, and 2 A. genomospecies 14TU. After 212 isolates were tested with the default Bruker database, the profiles of 63 additional Acinetobacter strains were added to the default database, and 517 isolates from 32 hospitals were assayed for validation. All strains in this study were confirmed by rpoB sequencing.
Results: The addition of the 63 Acinetobacter strains’ profiles to the default Bruker database increased the overall concordance rate between MALDI-TOF MS and rpoB sequencing from 69.8% (148/212) to 100.0% (517/517). Moreover, after library modification, all previously mismatched 64 Acinetobacter strains were correctly identified.
Conclusions: MALDI-TOF MS enables the prompt and accurate identification of clinically significant Acinetobacter species when used with the improved database.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Espinal P / 2011 / Clinical impact and molecular basis of antimicrobial resistance in non-baumannii Acinetobacter / Future Microbiol 6:495~511
Kishii K / 2014 / Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of Acinetobacter strains isolated from blood cultures / Clin Microbiol Infect 20:424~430
Lockhart SR / 2007 / Antimicrobial resistance among Gram-negative bacilli causing infections in intensive care unit patients in the United States between 1993 and 2004 / J Clin Microbiol 45:3352~3359
Kempf M / 2012 / Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options / Int J Antimicrob Agents 39:105~114
Fournier PE / 2006 / The epidemiology and control of Acinetobacter baumannii in health care facilities / Clin Infect Dis 42:692~699
Kishii K / 2014 / Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of Acinetobacter strains isolated from blood cultures / Clin Microbiol Infect 20:424~430
Lockhart SR / 2007 / Antimicrobial resistance among Gram-negative bacilli causing infections in intensive care unit patients in the United States between 1993 and 2004 / J Clin Microbiol 45:3352~3359
Kempf M / 2012 / Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options / Int J Antimicrob Agents 39:105~114
Fournier PE / 2006 / The epidemiology and control of Acinetobacter baumannii in health care facilities / Clin Infect Dis 42:692~699
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