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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 578 - 585 (8page)
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초록· 키워드
오류제보하기
Background: Conventional screening for congenital adrenal hyperplasia (CAH) using immunoassays generates a large number of false-positive results. A more specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been introduced to minimize unnecessary follow-ups. However, because of limited data on its use in the Korean population, LC-MS/MS has not yet been incorporated into newborn screening programs in this region. The present study aims to develop and validate an LC-MS/MS method for the simultaneous determination of seven steroids in dried blood spots (DBS) for CAH screening, and to define age-specific reference intervals in the Korean population.
Methods: We developed and validated an LC-MS/MS method to determine the reference intervals of cortisol, 17-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, corticosterone, and 11-deoxycorticosterone simultaneously in 453 DBS samples. The samples were from Korean subjects stratified by age group (78 full-term neonates, 76 premature neonates, 89 children, and 100 adults).
Results: The accuracy, precision, matrix effects, and extraction recovery were satisfactory for all the steroids at three concentrations; values of intra- and inter-day precision coefficients of variance, bias, and recovery were 0.7-7.7%, -1.5-9.8%, and 49.3-97.5%, respectively. The linearity range was 1-100 ng/mL for cortisol and 0.5-50 ng/mL for other steroids (R2>0.99). The reference intervals were in agreement with the previous reports.
Conclusions: This LC-MS/MS method and the reference intervals validated in the Korean population can be successfully applied to analyze seven steroids in DBS for the diagnosis of CAH.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ / Mayo medical laboratories reference laboratory services for health care organizations
Forest MG / 2004 / Recent advances in the diagnosis and management of congenital adrenal hyperplasia due to 21-hydroxylase deficiency / Hum Reprod Update 10 : 469 ~ 485
Hingre RV / 1994 / Adrenal steroidogenesis in very low birth weight preterm infants / J Clin Endocrinol Metab 78 : 266 ~ 270
Holst JP / 2007 / Use of steroid profiles in determining the cause of adrenal insufficiency / Steroids 72 : 71 ~ 84
Horowitz GL / 2008 / Defining, establishing, and verifying reference intervals in the clinical laboratory : approved guideline / Clinical and Laboratory Standards Institute
Forest MG / 2004 / Recent advances in the diagnosis and management of congenital adrenal hyperplasia due to 21-hydroxylase deficiency / Hum Reprod Update 10 : 469 ~ 485
Hingre RV / 1994 / Adrenal steroidogenesis in very low birth weight preterm infants / J Clin Endocrinol Metab 78 : 266 ~ 270
Holst JP / 2007 / Use of steroid profiles in determining the cause of adrenal insufficiency / Steroids 72 : 71 ~ 84
Horowitz GL / 2008 / Defining, establishing, and verifying reference intervals in the clinical laboratory : approved guideline / Clinical and Laboratory Standards Institute
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