지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 358 - 361 (4page)
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초록· 키워드
오류제보하기
Lymphocyte subset analysis is widely used in clinical laboratories, and more than two levels of daily QC materials are required for reliable results. Commercially available, expensive QC materials have short shelf lives and may not be suitable in resource-poor settings. We compared different methods for preparing homemade QC material, including fixation with 1%, 2%, or 4% paraformaldehyde (PFA); freezing with 10% dimethylsulfoxide (DMSO), 0.1% bovine serum albumin-phosphate buffered saline, or after ethanolic dehydration; and using cryopreservation temperatures of -20°C, -80°C, or -196°C. We found an optimal experimental condition, which is ‘fixation with 4% PFA, freezing with 10% DMSO, and storage at 80°C’. To evaluate long-term stability of QC materials prepared in this optimal condition, two levels of QC materials (QM1 and QM2) were thawed after 30, 33, 35, 37, 60, 62, 64, and 67 days of cryopreservation. Lymphocyte subset was analyzed with BD Multitest IMK kit (BD Biosciences, USA). QM1 and QM2 were stable after 1-2 months of cryopreservation (CV <3% for CD3, CD4, and CD8 and 5-7% for CD16/56 and CD19). We propose this method as an alternative cost-effective protocol for preparing homemade internal QC materials for lymphocyte subset analysis in resource-poor settings.
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등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
Mandy FF / 2003 / Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with human immunodeficiency virus. Centers for Disease Control and Prevention / MMWR Recomm Rep 52:1~13
Abdallah KO / 2006 / B cell monitoring of transplant patients treated with anti-CD20 / Clin Transpl :427~437
Wood B / 2013 / Validation of cell-based fluorescence assays: practice guidelines from the ICSH and ICCS - part V - assay performance criteria / Cytometry B Clin Cytom 84:315~323
Westerman LE / 2010 / A quality management systems approach for CD4 testing in resource-poor settings / Am J Clin Pathol 134:556~567
Fisher Scientific / BD Multi-Check Control Web Catalogue
Abdallah KO / 2006 / B cell monitoring of transplant patients treated with anti-CD20 / Clin Transpl :427~437
Wood B / 2013 / Validation of cell-based fluorescence assays: practice guidelines from the ICSH and ICCS - part V - assay performance criteria / Cytometry B Clin Cytom 84:315~323
Westerman LE / 2010 / A quality management systems approach for CD4 testing in resource-poor settings / Am J Clin Pathol 134:556~567
Fisher Scientific / BD Multi-Check Control Web Catalogue
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