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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 43 - 51 (9page)
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Enzyme/prodrug approach is one of the actively developing areas for cancer therapy. In an effort to develop more efective enzyme/prodrug sys-tems, cel-permeable cytosine deaminase was pro-duced by fusing yeast cytosine deaminase (yCD) which is an eficient delivery peptide of the foreign proteins into cels. The purified Tat-yCD fusion protein expresed in Escherichia coli was readily transduced into mamalian cels in a time- and dose-dependent manner. A significant level of the transduced Tat-yCD protein was recovered in the cel and was stable for 24 h as indicated by both results of the enzymatic assay of 5-fluorocytosine (5-FC) conversion to 5-fluorouracil (5-FU) and Western blot analysis. The cels transduced with toxicity of 5-FC, while cels treated with yCD are unafected by 5-FC. In addition, a strong bystander effect was observed with conditioned media from cells transduced with Tat-yCD added to non-trans-duced cels. Tat-yCD fusion protein demonstrated here for its ability to transduce into cells and con-vert nontoxic prodrug 5-FC to the toxic antimeta-bolite 5-FU, may be a useful approach for cancer therapy.
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참고문헌 (29)
참고문헌 신청
/ / A rapid and sensitive method for the quantitationof microgram quantities of protein utilizing the principleof protein-dye binding : 248 ~ 54
/ / Generation of 5-fluorouracil from 5-fluorocytosine by monoclonal antibody-cytosine deaminaseconjugates : 447 ~ 51
/ / Implantable enzymecapsules for cancer chemotherapy from bakers' yeast cytosinedeaminase immobilized on epoxy-acrylic resin and urethaneprepolymer to (mura k) : 61 ~ 9
/ / Intratumoral generation of 5-fluorouracil mediatedby an antibody-cytosine deaminase conjugate in combinationwith 5-fluorocytosine : 2719 ~ 23
/ / Tat-mediated delivery of heterologous proteinsinto cells : 664 ~ 8
/ / Generation of 5-fluorouracil from 5-fluorocytosine by monoclonal antibody-cytosine deaminaseconjugates : 447 ~ 51
/ / Implantable enzymecapsules for cancer chemotherapy from bakers' yeast cytosinedeaminase immobilized on epoxy-acrylic resin and urethaneprepolymer to (mura k) : 61 ~ 9
/ / Intratumoral generation of 5-fluorouracil mediatedby an antibody-cytosine deaminase conjugate in combinationwith 5-fluorocytosine : 2719 ~ 23
/ / Tat-mediated delivery of heterologous proteinsinto cells : 664 ~ 8
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