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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제5호
- 발행연도
- 2008.1
- 수록면
- 852 - 858 (7page)
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초록· 키워드
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An extracellular protease (Mc1) was isolated from the
nematode-trapping fungus Monacrosporium cystosporium
by gel filtration, anion-exchange, and hydrophobic interaction
chromatographies. This protease had a molecular mass of
approximately 38 kDa and displayed an optimal activity
at pH 7-9 and 56oC (over 30 min). Its proteolytic activity
was highly sensitive to the serine protease inhibitor PMSF
(phenylmethylsulfonylfluoride, 0.1 mM), indicating that it
belonged to the serine-type peptidase group. The Michaelis
constant (Km) and Vmax for substrate N-Suc-Ala-Ala-Pro-
Phe-pNA were 1.67×10-4 M and 0.6071 OD410 per 30 s,
respectively. This protease could degrade a broad range of
substrates including casein, gelatin, BSA (bovine serum
albumin), and nematode cuticle. Moreover, the enzyme could
immobilize the free-living nematode Panagrellus redivivus
and the pine wood nematode Bursaphelenchus xylophilus,
suggesting that it might play a role in infection against
nematodes. The encoding gene of Mc1 was composed of
one intron and two exons, coding for a polypeptide of 405
amino acid residues. The deduced amino acid sequence of
Mc1 showed 61.4-91.9% identity to serine proteases from
other nematode-trapping fungi. Our results identified that
Mc1 possessed biochemical properties including optimal
reaction condition and substrate preference that are different
from previously identified serine proteases.
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참고문헌 (23)
참고문헌 신청
Ahman, J. / 1996 / Sequence analysis and regulation of a gene encoding a cuticle-degrading serine protease from the nematophagous fungus Arthrobotrys oligospora / Microbiolog
Betzel, C. / 1988 / Three-dimensional structure of proteinase K at 0.15 nm resolution. Eur / Biochem 178 : 155 ~ 171
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal / Biochem
Cox, G. N. / 1981 / Cuticle of Caenorhabditis elegans: Its isolation and partial characterization / Cell Biol 90 : 7 ~ 17
Dong, J. Y. / 2004 / Nematicidal effect of freshwater fungal cultures against the pine-wood nematode, Bursaphelenchus xylophilus / Fungal Divers 15 : 123 ~ 133
Betzel, C. / 1988 / Three-dimensional structure of proteinase K at 0.15 nm resolution. Eur / Biochem 178 : 155 ~ 171
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal / Biochem
Cox, G. N. / 1981 / Cuticle of Caenorhabditis elegans: Its isolation and partial characterization / Cell Biol 90 : 7 ~ 17
Dong, J. Y. / 2004 / Nematicidal effect of freshwater fungal cultures against the pine-wood nematode, Bursaphelenchus xylophilus / Fungal Divers 15 : 123 ~ 133
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