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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제3호
- 발행연도
- 2016.1
- 수록면
- 498 - 502 (5page)
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초록· 키워드
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3’-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3’-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3’-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3’- hydroxygenistein, indicating a high potential for industrial-scale 3’-hydroxygenistein production.
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참고문헌 (16)
참고문헌 신청
Chang TS / 2014 / Isolation, bioactivity, and production of ortho-hydroxydaidzein and ortho-hydroxygenistein / Int. J. Mol. Sci 15:5699~5716
Charaux C / 1939 / Constitution chimique de l'orobol / Bull. Soc. Chim. Biol. (Paris) 21:1330~1333
Chen QC / 2010 / Flavonoids and isoflavonoids from Sophorae Flos improve glucose uptake in vitro / Planta Med 76:79~81
Ding HY / 2015 / Identifying 3'-hydroxygenistein as potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris / Process Biochem 50:1614~1617
Ding HY / 2014 / Methanol partition fraction of ethanol extract of Dioscorea nipponica Makino inhibits melanogenesis / Trop. J. Pharm. Res 13:719~726
Charaux C / 1939 / Constitution chimique de l'orobol / Bull. Soc. Chim. Biol. (Paris) 21:1330~1333
Chen QC / 2010 / Flavonoids and isoflavonoids from Sophorae Flos improve glucose uptake in vitro / Planta Med 76:79~81
Ding HY / 2015 / Identifying 3'-hydroxygenistein as potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris / Process Biochem 50:1614~1617
Ding HY / 2014 / Methanol partition fraction of ethanol extract of Dioscorea nipponica Makino inhibits melanogenesis / Trop. J. Pharm. Res 13:719~726
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