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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제6호
- 발행연도
- 2008.1
- 수록면
- 1,101 - 1,108 (8page)
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Geldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells.
To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
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참고문헌 (21)
참고문헌 신청
August, P. R. / 1998 / Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis medite
Becker, A. M. / 1983 / 3-Amino-5-hydroxybenzoic acid in antibiotic biosynthesis. VI. Directed biosynthesis studies with ansamycin antibiotics / Antibiot 36 : 1323 ~ 1328
Bibb, M. J. / 1994 / The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-bindi
Bierman, M. / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Cupp-Vickery, J. R. / 1995 / Structure of cytochrome P450eryF involved in erythromycin biosynthesis. Nat. Struct / Biol 2 : 144 ~ 153
Becker, A. M. / 1983 / 3-Amino-5-hydroxybenzoic acid in antibiotic biosynthesis. VI. Directed biosynthesis studies with ansamycin antibiotics / Antibiot 36 : 1323 ~ 1328
Bibb, M. J. / 1994 / The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-bindi
Bierman, M. / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Cupp-Vickery, J. R. / 1995 / Structure of cytochrome P450eryF involved in erythromycin biosynthesis. Nat. Struct / Biol 2 : 144 ~ 153
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