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자료유형
학술저널
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저널정보
대한안과학회 Korean Journal of Ophthalmology Korean Journal of Ophthalmology 제32권 제2호
발행연도
2018.1
수록면
147 - 153 (7page)

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Interleukin (IL)-22 is a cytokine involved in epithelial cell regeneration. Currently, no research studieshave analyzed the distribution of the three distinct IL-22–secreting cell populations in human or mouse conjunctiva. This study investigated the distribution of the three main populations of IL-22–secreting immune cells, αβ Thcells, γδ T cells, or innate cells (innate lymphoid cells [ILCs] or natural killer cells), in conjunctival associated lymphoidtissues (CALTs) in human and mouse models. Methods: We collected discarded cadaveric bulbar conjunctival tissue specimens after preservation of the corneo-limbal tissue for keratoplasty from four enucleated eyes of the domestic donor. The bulbar conjunctivatissue, including the cornea from normal (n = 27) or abraded (n = 4) B6 mice, were excised and pooled in RPMI1640 media. After the lymphoid cells were gated in forward and side scattering, the αβ Th cells, γδ T cells, orinnate lymphoid cells were positively or negatively gated using anti-CD3, anti-γδ TCR, and anti–IL-22 antibodies,with a FACSCanto flow cytometer. Results: In normal human conjunctiva, the percentage and number of cells were highest in αβ Th cells, followed byγδ T cells and CD3– γδ TCR – IL-22+ innate cells (presumed ILCs, pILCs) (Kruskal-Wallis test, p = 0.012). In normalmice keratoconjunctiva, the percentage and total number were highest in γδ T cells, followed by αβ Th cellsand pILCs (Kruskal-Wallis test, p = 0.0004); in corneal abraded mice, the population of αβ Th cells and pILCstended to increase. Conclusions: This study suggests that three distinctive populations of IL-22–secreting immune cells are presentin CALTs of both humans and mice, and the proportions of IL-22+αβ Th cells, γδ T cells, and pILCs in CALTs inhumans might be differently distributed from those in normal mice.

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