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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제2호
- 발행연도
- 2019.1
- 수록면
- 304 - 310 (7page)
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초록· 키워드
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Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.
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참고문헌 (41)
참고문헌 신청
Rochman Y / 2009 / New insights into the regulation of T cells by gamma(c)family cytokines / Nat. Rev. Immunol 9:480~490
Spolski R / 2014 / Interleukin-21 : a doubleedged sword with therapeutic potential / Nat. Rev. Drug Discov 13:379~395
Asao H / 2001 / Cutting edge : the common γ-chain is an indispensable subunit of the IL-21 receptor complex / J. Immunol 167:1~5
강상민 / 2017 / Host Innate Immunity against Hepatitis E Virus and Viral Evasion Mechanisms / Journal of Microbiology and Biotechnology 27(10):1727~1735
강상민 / 2017 / Primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas / The Journal of Microbiology 55(5):319~329
Spolski R / 2014 / Interleukin-21 : a doubleedged sword with therapeutic potential / Nat. Rev. Drug Discov 13:379~395
Asao H / 2001 / Cutting edge : the common γ-chain is an indispensable subunit of the IL-21 receptor complex / J. Immunol 167:1~5
강상민 / 2017 / Host Innate Immunity against Hepatitis E Virus and Viral Evasion Mechanisms / Journal of Microbiology and Biotechnology 27(10):1727~1735
강상민 / 2017 / Primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas / The Journal of Microbiology 55(5):319~329
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