지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 221 - 226 (6page)
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초록· 키워드
오류제보하기
Background: Intestinal protozoan infection is one of the main causes of gastrointestinal diseases. Protozoa are usually detected by direct smear microscopy, concentration techniques, or special stains; however, these techniques are labor-intensive and require well-trained technicians. Therefore, molecular techniques involving polymerase chain reaction (PCR) have been developed to satisfy the need for unbiased and rapid analytical methods with high sensitivity and specificity. In this study, the BD MAXTM Enteric Parasite Panel (EPP) (Becton, Dickinson and Company, USA), designed to detect Cryptosporidium parvum and/or hominis, Giardia lamblia, and Entamoeba histolytica, and the AllplexTM Gastrointestinal Parasite Assays (AGPA) (Seegene Inc., Korea), designed to detect Cryptosporidium species, G. lamblia, E. histolytica, Blastocystis hominis, Dientamoeba fragilis, and Cyclospora cayetanensis were compared to determine whether any of these assays could become a useful tool for detecting intestinal protozoan infections in Korea. Methods: We investigated 295 fecal samples using EPP and AGPA. Then we confirmed the positive results with the conventional and nested PCR. Consistent detection by conventional PCR, nested PCR, and one of the multiplex panels was considered “true positive.”Results: Out of 295 samples, 17 were true positives for B. hominis and 2 were true positives for E. histolytica. EPP detected parasites in only two samples owing to its design; however, its true positive detection rate was 100% (2/2). AGPA detected parasites in 24 samples with 79.2% (19/24) true positives. Conclusions: The incidence of protozoan, especially B. hominis, infection may be more prevalent than expected. AGPA could be an effective tool for screening protozoan infections.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Fletcher SM / 2012 / Enteric protozoa in the developed world : a public health perspective / Clin Microbiol Rev 25:420~449
Won EJ / 2016 / Multiplex real-time PCR assay targeting eight parasites customized to the Korean population : Potential use for detection in diarrheal stool samples from gastroenteritis patients / PLoS One 11:e0166957
Autier B / 2018 / Comparison of three commercial multiplex PCR assays for the diagnosis of intestinal protozoa / Parasite 25:48
Ocaña-Losada C / 2018 / Clinical and epidemiological characteristics of intestinal parasite infection by Blastocystis hominis / Rev Clin Esp 218:115~120
Mergani MH / 2014 / Detection of intestinal protozoa by using different methods / Dent Med Res 2:28~32
Won EJ / 2016 / Multiplex real-time PCR assay targeting eight parasites customized to the Korean population : Potential use for detection in diarrheal stool samples from gastroenteritis patients / PLoS One 11:e0166957
Autier B / 2018 / Comparison of three commercial multiplex PCR assays for the diagnosis of intestinal protozoa / Parasite 25:48
Ocaña-Losada C / 2018 / Clinical and epidemiological characteristics of intestinal parasite infection by Blastocystis hominis / Rev Clin Esp 218:115~120
Mergani MH / 2014 / Detection of intestinal protozoa by using different methods / Dent Med Res 2:28~32
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