Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea

Seobo Sim; Jua Won; Jae-Whan Kim; Kyungjin Kim; Woo-Yoon Park; Jae-Ran Yu

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저자정보: Seobo Sim (Konkuk University School of Medicine) Jua Won (Konkuk University School of Medicine) Jae-Whan Kim (Konkuk University School of Medicine) Kyungjin Kim (Konkuk University School of Medicine) Woo-Yoon Park (Chungbuk National University) Jae-Ran Yu (Konkuk University School of Medicine)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.2

발행연도: 2017.4

수록면: 137 - 142 (6page)

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Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragmentation and nested PCR confirmed Cryptosporidium parvum and Cyclospora cayetanensis, respectively. In conclusion, we detected C. cayetanensis in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea.

#Cryptosporidium parvum #Cyclospora cayetanensis #vegetable #real-time PCR #Korea

참고문헌 (30)

참고문헌 신청

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[학술지(정기간행물)] - Ortega YR / 1998 / Cyclospora cayetanensis / Adv Parasitol 40 : 399 ~ 418

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