지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 631 - 636 (6page)
이용수
초록· 키워드
오류제보하기
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively. The multiplex-touchdown PCR protocol using a primer mixture simultaneously detected protozoa in human stools, and the amplified gene was detected in >1×10³ oocysts for C. parvum, >1×10⁴ cysts for G. lamblia, and >1 copy of the 18S rRNA gene for C. cayetanensis. Taken together, our protocol convincingly demonstrated the ability to simultaneously detect C. parvum, G. lamblia, and C. cayetanenesis in stool samples.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Diemert DJ / 2006 / Prevention and self-treatment of traveler's diarrhea / Clin Microbiol Rev 19 : 583 ~ 594
[학술지(정기간행물)] - DuPont HL / 2006 / Travellers’ diarrhoea : contemporary approaches to therapy and prevention / Drugs 66 : 303 ~ 314
[학술지(정기간행물)] - Wood AJ / 1993 / Prevention and treatment of traveler's diarrhea / New Engl J Med 328 : 1821 ~ 1827
[단행본] - Bradley AC / 2016 / CDC Health Information for International Travel 2016 / Oxford University Press
[학술지(정기간행물)] - Freedman DO / 2006 / Spectrum of disease and relation to place of exposure among ill returned travelers / New Engl J Med 354 : 119 ~ 130
[학술지(정기간행물)] - DuPont HL / 2006 / Travellers’ diarrhoea : contemporary approaches to therapy and prevention / Drugs 66 : 303 ~ 314
[학술지(정기간행물)] - Wood AJ / 1993 / Prevention and treatment of traveler's diarrhea / New Engl J Med 328 : 1821 ~ 1827
[단행본] - Bradley AC / 2016 / CDC Health Information for International Travel 2016 / Oxford University Press
[학술지(정기간행물)] - Freedman DO / 2006 / Spectrum of disease and relation to place of exposure among ill returned travelers / New Engl J Med 354 : 119 ~ 130
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples
Parasites, Hosts and Diseases
2018 .10
-
Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Parasites, Hosts and Diseases
2017 .04
-
Outbreak of Cyclosporiasis in Korean Travelers Returning from Nepal
Parasites, Hosts and Diseases
2020 .10
-
Monitoring of Noxious Protozoa for Management of Natural Water Resources
Parasites, Hosts and Diseases
2018 .04
-
Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia
Parasites, Hosts and Diseases
2020 .12
-
Occurrence of Cryptosporidium and Giardia and the Relationship between Protozoa and Water Quality Indicators in Swimming Pools
Parasites, Hosts and Diseases
2017 .04
-
Increased Innate Lymphoid Cell 3 and IL-17 Production in Mouse Lamina Propria Stimulated with Giardia lamblia
Parasites, Hosts and Diseases
2019 .06
-
비전형적인 증상의 람블편모충 감염에 의한 대장염 1예
대한내과학회지
2015 .01
-
Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified
Parasites, Hosts and Diseases
2020 .12
-
An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting
Parasites, Hosts and Diseases
2016 .02
-
Erratum : Laboratory Aspects of Donor Screening for Fecal Microbiota Transplantation at a Korean Fecal Microbiota Bank
Annals of Laboratory Medicine
2021 .11
-
Molecular Identification of Cryptosporidium Species from Pet Snakes in Thailand
Parasites, Hosts and Diseases
2016 .08
-
Identification of a Novel Microtubule-Binding Protein in Giardia lamblia
Parasites, Hosts and Diseases
2016 .08
-
RNA-sequencing Profiles of Cell Cycle–Related Genes Upregulated during the G2-Phase in Giardia lamblia
Parasites, Hosts and Diseases
2019 .04
-
Alteration of goat kids’ gut microbiota by Cryptosporidium parvum infection
Journal of Biomedical and Translational Research
2024 .06
-
Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
Parasites, Hosts and Diseases
2019 .10
-
Internal Amplification Control for a Cryptosporidium Diagnostic PCR : Construction and Clinical Evaluation
Parasites, Hosts and Diseases
2015 .04
-
Performance validation of the BD MAX Enteric Parasite Panel using simulated samples in low endemic regions
Parasites, Hosts and Diseases
2025 .02
-
Detection of Intestinal Protozoa in Korean Patients Using BD MAX Enteric Parasite Panel and Seegene Allplex Gastrointestinal Parasite Assay
Laboratory Medicine Online
2020 .01
-
Comparison of blood electrolyte and biochemical parameters between single infections of rotavirus and Cryptosporidium parvum in diarrheic Hanwoo calves
Journal of Veterinary Science
2022 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-513-001586493