지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The steroid 9${alpha}$-hydroxylase activity has been detected in cytosol fraction, $100,00{\times}g$ supernatant of cell free extract of Mycobacterium fortuitum. The activity was not linear with protein concentration in the assay suggesting 9${alpha}$-hydroxylase is a multicomponent enzyme. The 9${alpha}$-hydroxylase system was partially purified through fractional saturation of ammonium sulfate, strong anion exchange (Mono Q) column chromatography, gel filtration (Superose 12) column chromatography, and testosterone affinity gel chromatography. Ammonium sulfate 50~60% saturated fraction of the cytosol gave 9${alpha}$-hydroxylase activity. For further purification, the half-saturated ammonium sulfate fraction was applied to Mono Q, Superose 12, or affinity gel column. The purification factors of 9${alpha}$-hydroxylase containing fraction after Mono Q, Superose 12, and affinity gel chromatography was 13, 11, and 17 respectively.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Heterogeneous Natures of the Microbial Steroid $9{\alpha}$-Hydroxylase in Nocardioforms
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1997 .01
-
A case of 17 alpha-hydroxylase deficiency
Clinical and experimental reproductive medicine : CERM
2015 .01
-
Microbial $9{\alpha}$-Hydroxylase:Epoxidation of 9(11)-dehydro-$17{\alpha}$-methyl-testosterone
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1997 .01
-
Purification of the NADH Reductase Component of the Steroid $9{\alpha}$-hydroxylase from Mycobacterium fortuitum
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1997 .01
-
EFFECT OF MONOSODIUM GLUTAMATE ON BRAIN TYROSINE HYDROXYLASE, DOPAMINE-β-HYDROXYLASE, TRYPTOPHAN HYDROXYLASE AND MONOAMINE OXIDASE IN RAT
한국독성학회지
1987 .06
-
흰쥐에서 에탄올이 유도한 간 Aniline Hydroxylase활성에 미치는 인삼의 영향 ( Effects of Ginseng Butanol Fraction on Ethanol - Induced Hepatic Hydroxylase Activity in Rat )
Journal of Ginseng Research
1985 .12
-
New inhibitors of the NF-kB activation and NO production from Artemisia sylvatica
대한약학회 학술대회
2003 .01
-
Enzymatic Study on Acetanilide p-Hydroxylase in Streptomyces fradiae
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1992 .01
-
17α-hydroxylase 결핍증에 의한 원발성 무월경 1예
대한내과학회지
2002 .01
-
Possible target for G protein antagonist: Identification of specific amino acid residue responsible for the molecular interaction of G$\alpha$ 16 with chemoattractant C5a receptor.
한국응용약물학회 춘계학술발표논문집
2000 .01
-
Recent advances in biochemical and molecular analysis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency
Annals of Pediatirc Endocrinology & Metabolism
2016 .01
-
Gel filtration chromatography와 propionic acid/urea polyacrylamide gel electrophoresis를 이용한 봉독 성분의 분리
Journal of Pharmacopuncture
2006 .01
-
Vitamin D dependent rickets type I
Clinical and Experimental Pediatrics
2011 .01
-
An Efficient Synthesis of Cyclic $\alpha$, $\alpha$-Disubstituted $\alpha$-Amino Acids
대한약학회 학술대회
2001 .01
-
17α-hydroxylase 결핍으로 인한 고혈압을 동반한 원발성 무월경 1예
Obstetrics & Gynecology Science
2007 .01
-
In Vivo Suppression of Bisphenol A on Estradiol 2- and 4-Hydroxylase Activities in Hepatic Microsomal Fractions of Male and Female Sprague-Dawley Rats
Biomolecules & Therapeutics(구 응용약물학회지)
2009 .01
-
Mycobacterium fortuitum에 의한 척추골수염 및 경막외 농양 1예
대한내과학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0