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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Sejeong (Department of Food and Nutrition, Sookmyung Women's University) Lee, Soomin (Department of Food and Nutrition, Sookmyung Women's University) Lee, Heeyoung (Department of Food and Nutrition, Sookmyung Women's University) Ha, Jimyeong (Department of Food and Nutrition, Sookmyung Women's University) Lee, Jeeyeon (Department of Food and Nutrition, Sookmyung Women's University) Choi, Yukyung (Department of Food and Nutrition, Sookmyung Women's University) Oh, Hyemin (Department of Food and Nutrition, Sookmyung Women's University) Hong, Joonbae (Test & Research Department, Consumer Safety Center, Korea Consumer Agency) Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University) Choi, Kyoung-Hee (Department of Oral Microbiology, College of Dentistry, Wonkwang University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제37권 제4호
발행연도
2017.1
수록면
502 - 510 (9page)

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This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were $8{\mu}g/mL$ for Streptococcus mutans, and $16{\mu}g/mL$ for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration $8-16{\mu}g/mL$. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.

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