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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 867 - 873 (7page)
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초록· 키워드
오류제보하기
1,4-Dihydroxy-2-naphthoic acid (DHNA), a precursor of menaquinone (vitamin K2), has an effect on growth stimulation of bifidobacteria and prevention of osteoporosis, making it a promising functional food material. Therefore, we tried to clone the menB gene encoding DHNA synthase from Leuconostoc mesenteroides CJNU 0147. Based on the genome sequence of Leu. mesenteroides ATCC 8293 (GenBank accession no., CP000414), a primer set (Leu_menBfull_F and Leu_menBfull_R) was designed for the PCR amplification of menB gene of CJNU 0147. A DNA fragment (1,190 bp), including the menB gene, was amplified, cloned into pGEM-T Easy vector, and sequenced. The deduced amino acid sequence of MenB (DHNA synthase) protein of CJNU 0147 had a 98% similarity to the corresponding protein of ATCC 8293. The menB gene was subcloned into pCW4, a lactic acid bacteria - E. coli shuttle vector, and transferred to CJNU 0147. The transcription of menB gene of CJNU 0147 (pCW4::menB) was increased, when compared with those of CJNU 0147 (pCW4) and CJNU 0147 (−). The DHNA was produced from it at a detectable level, indicating that the cloned menB gene of CJNU 0147 encoded a DHNA synthase which is responsible for the production of DHNA, resulting in an increase of bifidogenic growth stimulation activity.
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참고문헌 (32)
참고문헌 신청
Aggarwal, J. / 2013 / Probiotics and their effects on metabolic diseases: An update / J. Clin. Diagn. Res 7:173~177
An, H. M. / 2010 / Efficacy of lactic acid bacteria (LAB) supplement in management of constipation among nursing home residents / Nutr. J 9:5
Chen, T. / 2014 / Microbiological quality and characteristics of probiotic products in China / J. Sci. Food Agric 94:131~138
Chung, C. H. / 2002 / Glucooligosaccharides from Leuconostoc mesenteroides B-742 (ATCC 13146): a potential prebiotic / J. Ind. Microbiol. Biotechnol 29:196~199
Chung, C. H. / 2004 / Efficacy of Leuconostoc mesenteroides (ATCC 13146) isomaltooligosaccharides as a poultry prebiotic / Poultry Sci 83:1302~1306
An, H. M. / 2010 / Efficacy of lactic acid bacteria (LAB) supplement in management of constipation among nursing home residents / Nutr. J 9:5
Chen, T. / 2014 / Microbiological quality and characteristics of probiotic products in China / J. Sci. Food Agric 94:131~138
Chung, C. H. / 2002 / Glucooligosaccharides from Leuconostoc mesenteroides B-742 (ATCC 13146): a potential prebiotic / J. Ind. Microbiol. Biotechnol 29:196~199
Chung, C. H. / 2004 / Efficacy of Leuconostoc mesenteroides (ATCC 13146) isomaltooligosaccharides as a poultry prebiotic / Poultry Sci 83:1302~1306
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