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논문 기본 정보

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학술저널
저자정보
Sanjib Kumar Sardar (National Institute of Cholera and Enteric Diseases) Ajanta Ghosal (National Institute of Cholera and Enteric Diseases) Yumiko Saito-Nakano (National Institute of Infectious Diseases) Shanta Dutta (ICMR-National Institute of Cholera and Enteric Diseases) Tomoyoshi Nozaki (The University of Tokyo) Sandipan Ganguly (National Institute of Cholera and Enteric Diseases)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.59 No.4
발행연도
2021.8
수록면
409 - 413 (5page)

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In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally dis-tributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.

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