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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 15 - 22 (8page)
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Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and BclxL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.
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참고문헌 (26)
참고문헌 신청
Lee HJ / 2008 / Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced apoptosis / Toxicology 250:109~115
Zhang Y / 2000 / Oxidative stress and genetics in the pathogenesis of Parkinson’s disease / Neurobiol Dis 7:240~250
Jenner P / 2003 / Oxidative stress in Parkinson’s disease / Ann Neurol 53(Suppl 3):S26~S36
Wang W / 2008 / Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells / Cell Mol Neurobiol 28:293~305
Ziegler D / 1999 / Alpha-lipoic acid in the treatment of diabetic polyneuropathy in Germany : current evidence from clinical trials / Exp Clin Endocrinol Diabetes 107:421~430
Zhang Y / 2000 / Oxidative stress and genetics in the pathogenesis of Parkinson’s disease / Neurobiol Dis 7:240~250
Jenner P / 2003 / Oxidative stress in Parkinson’s disease / Ann Neurol 53(Suppl 3):S26~S36
Wang W / 2008 / Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells / Cell Mol Neurobiol 28:293~305
Ziegler D / 1999 / Alpha-lipoic acid in the treatment of diabetic polyneuropathy in Germany : current evidence from clinical trials / Exp Clin Endocrinol Diabetes 107:421~430
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