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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 191 - 202 (12page)
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Purpose: A near-infrared (NIR) fluorescence imaging is a promising tool for cancer-specific image guided surgery. Human epidermal receptor 2 (HER2) is one of the candidate markers for gastric cancer. In this study, we aimed to synthesize HER2-specific NIR fluorescence probes and evaluate their applicability in cancer-specific image-guided surgeries using an animal model. Materials and Methods: An NIR dye emitting light at 800 nm (IRDye800CW; Li-COR) was conjugated to trastuzumab and an HER2-specific affibody using a click mechanism. HER2 affinity was assessed using surface plasmon resonance. Gastric cancer cell lines (NCI-N87 and SNU-601) were subcutaneously implanted into female BALB/c nu (6?8 weeks old) mice. After intravenous injection of the probes, biodistribution and fluorescence signal intensity were measured using Lumina II (Perkin Elmer) and a laparoscopic NIR camera (InTheSmart). Results: Trastuzumab-IRDye800CW exhibited high affinity for HER2 (KD=2.093(3) pM). Fluorescence signals in the liver and spleen were the highest at 24 hours post injection, while the signal in HER2-positive tumor cells increased until 72 hours, as assessed using the Lumina II system. The signal corresponding to the tumor was visually identified and clearly differentiated from the liver after 72 hours using a laparoscopic NIR camera. Affibody-IRDye800CW also exhibited high affinity for HER2 (KD=4.71 nM); however, the signal was not identified in the tumor, probably owing to rapid renal clearance. Conclusions: Trastuzumab-IRDye800CW may be used as a potential NIR probe that can be injected 2?3 days before surgery to obtain high HER2-specific signal and contrast. Affibody-based NIR probes may require modifications to enhance mobilization to the tumor site.
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참고문헌 (27)
참고문헌 신청
Diana M / 2017 / Superselective intra-arterial hepatic injection of indocyanine green(ICG)for fluorescence image-guided segmental positive staining : experimental proof of the concept / Surg Endosc 31:1451~1460
Huh YJ / 2019 / Efficacy of assessing intraoperative bowel perfusion with near-infrared camera in laparoscopic gastric cancer surgery / J Laparoendosc Adv Surg Tech A 29:476~483
Park SH / 2020 / Near-infrared fluorescence-guided surgery using indocyanine green facilitates secure infrapyloric lymph node dissection during laparoscopic distal gastrectomy / Surg Today 50:1187~1196
Connell CM / 2017 / Activating HER2 mutations as emerging targets in multiple solid cancers / ESMO Open 2:e000279
Tajima JY / 2018 / Clinical significance of glycoprotein non-metastatic B and its association with EGFR/HER2 in gastrointestinal cancer / J Cancer 9:358~366
Huh YJ / 2019 / Efficacy of assessing intraoperative bowel perfusion with near-infrared camera in laparoscopic gastric cancer surgery / J Laparoendosc Adv Surg Tech A 29:476~483
Park SH / 2020 / Near-infrared fluorescence-guided surgery using indocyanine green facilitates secure infrapyloric lymph node dissection during laparoscopic distal gastrectomy / Surg Today 50:1187~1196
Connell CM / 2017 / Activating HER2 mutations as emerging targets in multiple solid cancers / ESMO Open 2:e000279
Tajima JY / 2018 / Clinical significance of glycoprotein non-metastatic B and its association with EGFR/HER2 in gastrointestinal cancer / J Cancer 9:358~366
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