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논문 기본 정보

자료유형
학술저널
저자정보
Jian‑Cheng Zhai (Northeast Forestry University) Ruo‑Bing Han (Northeast Forestry University) Sheng‑Nan Wang (Northeast Forestry University) Qiang‑Hui Wang (Northeast Forestry University) Yan‑Ling Xia (Northeast Forestry University) Wei‑Shi Liu (Northeast Forestry University) Ya‑Jie Yin (Daqing Normal University) He‑Ping Li (Northeast Forestry University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.41 No.9
발행연도
2019.1
수록면
1,007 - 1,013 (7page)

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Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.
#Reindeer · DNA methylation · mRNA expression · COL6A3 · Bisulfite sequencing PCR

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참고문헌 (34)

참고문헌 신청
Barling PM / 2004 / Expression of PTHrP and the PTH/PTHrP receptor in growing red deer antler / Cell Biol Int 28:661~673 google schola Bubenik GA / 1987 / Developing antler, a model for endocrine regulation of bone growth. Concentration gradient of T3, T4, and alkaline phosphatase in the antler, jugular, and the saphenous veins / Calcif Tissue Int 41:38~43 google schola Clark DE / 2006 / Expression of VEGF and pleiotrophin in deer antler / Anat Rec Part A 288:1281~1293 google schola Esteller M / 2008 / Epigenetics in cancer / N Engl J Med 358:1148 google schola Fujita T / 2002 / The effect of active absorbable algal calcium(AAA Ca)with collagen and other matrix components on back and joint pain and skin impedance / J Bone Miner Metab 20:298~302 google schola

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