Topographic, histological and molecular study of aberrant crypt foci identified in human colon in different clinical groups

Shouriyo Ghosh; Brijnandan Gupta; Pavan Verma; Sreenivas Vishnubathla; Sujoy Pal; Nihar R Dash; Siddhartha Datta Gupta; Prasenjit Das

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자료유형: 학술저널

저자정보: Shouriyo Ghosh (All India Institute of Medical Sciences New Delhi India) Brijnandan Gupta (All India Institute of Medical Sciences New Delhi India) Pavan Verma (All India Institute of Medical Sciences New Delhi India) Sreenivas Vishnubathla (All India Institute of Medical Sciences New Delhi India) Sujoy Pal (All India Institute of Medical Sciences New Delhi India) Nihar R Dash (All India Institute of Medical Sciences New Delhi India) Siddhartha Datta Gupta (All India Institute of Medical Sciences New Delhi India) Prasenjit Das (All India Institute of Medical Sciences New Delhi India)

저널정보: 대한장연구학회 Intestinal research Intestinal research Vol.16 No.1

발행연도: 2018.1

수록면: 116 - 125 (10page)

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Background/Aims: Aberrant crypt foci (ACF) are early microscopic lesions of the colonic mucosa, which can be detected bymagnified chromoendoscopy. Herein, we have investigated whether ACF identified in different clinical groups can be differentiated based on their characteristics. Methods: Macroscopically unremarkable mucosal flaps were collected from 270 freshcolectomies and divided into 3 clinical groups: colorectal carcinoma (group A), disease controls having known pre-neoplasticpotential (group Bc), and disease controls without risk of carcinoma development (group Bn). Topographic and histologic anal-ysis, immunohistochemistry, and molecular studies (high-resolution melt curve analysis, real-time polymerase chain reaction,and Sanger sequencing) were conducted for certain neoplasia-associated markers. Results: ACF were seen in 107 cases, out ofwhich 72 were left colonic ACF and 35 right colonic ACF (67.2% vs. 32.7%, P =0.02). The overall density of left colonic ACF was0.97/cm, which was greater than the right colonic ACF density of 0.81/cm. Hypercrinia was present in 41 out of 72 left colonicACF and in 14 out of 35 right colonic ACF (P =0.01). Immunohistochemical expression of p53 was also greater in left colonicACF than in right colonic ACF (60.5% vs. 38.2%, P =0.03). However, ACF identified among the 3 clinical groups did not show anydistinguishing topographic, histological, or genetic changes. Conclusions: Left colonic ACF appear to be high-risk based ontheir morphological and prototypic tumor marker signature. ACF identified in different clinical groups do not show significantgenotypic or topographic differences. Further detailed genetic studies are required to elucidate them further.

참고문헌 (28)

참고문헌 신청

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Indian Council of Medical Research / 2010 / ICMR Bulletin / Indian Council of Medical Research

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