지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.11
- 수록면
- 846 - 854 (9page)
- DOI
- 10.14348/molcells.2022.0081
이용수
초록· 키워드
오류제보하기
Neurons make long-distance connections via their axons, and the accuracy and stability of these connections are crucial for brain function. Research using various animal models showed that the molecular and cellular mechanisms underlying the assembly and maintenance of neuronal circuitry are highly conserved in vertebrates. Therefore, to gain a deeper understanding of brain development and maintenance, an efficient vertebrate model is required, where the axons of a defined neuronal cell type can be genetically manipulated and selectively visualized in vivo. Placental mammals pose an experimental challenge, as time-consuming breeding of genetically modified animals is required due to their in utero development. Xenopus laevis, the most commonly used amphibian model, offers comparative advantages, since their embryos ex utero during which embryological manipulations can be performed. However, the tetraploidy of the X. laevis genome makes them not ideal for genetic studies. Here, we use Xenopus tropicalis, a diploid amphibian species, to visualize axonal pathfinding and degeneration of a single central nervous system neuronal cell type, the retinal ganglion cell (RGC). First, we show that RGC axons follow the developmental trajectory previously described in X. laevis with a slightly different timeline. Second, we demonstrate that co-electroporation of DNA and/or oligonucleotides enables the visualization of gene function-altered RGC axons in an intact brain. Finally, using this method, we show that the axon-autonomous, Sarm1-dependent axon destruction program operates in X. tropicalis. Taken together, the present study demonstrates that the visual system of X. tropicalis is a highly efficient model to identify new molecular mechanisms underlying axon guidance and survival.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
miRNAs-dependent regulation of synapse formation and function
Genes & Genomics
2020 .01
-
NELL2 Function in Axon Development of Hippocampal Neurons
Molecules and Cells
2020 .01
-
Epigenetic Regulation of Axon Regeneration after Neural Injury
Molecules and Cells
2017 .01
-
Beyond Protein Folding: Exploring the Role of Unfolded Protein Response in Axon Targeting
Molecules and Cells
2023 .06
-
Molecular Mechanisms Underlying Motor Axon Guidance in Drosophila
Molecules and Cells
2021 .08
-
Identification of cis-Regulatory Region Controlling Semaphorin-1a Expression in the Drosophila Embryonic Nervous System
Molecules and Cells
2020 .01
-
Function and regulation of nitric oxide signaling in Drosophila
Molecules and Cells
2024 .01
-
Targeted Base Editing via RNA-Guided Cytidine Deaminases in Xenopus laevis Embryos
Molecules and Cells
2017 .11
-
Neurons-on-a-Chip: In Vitro NeuroTools
Molecules and Cells
2022 .02
-
Candida tropicalis Isolated from Tuak, a North Sumatera- Indonesian Traditional Beverage, for Bioethanol Production
한국미생물·생명공학회지
2015 .01
-
Characteristics of the Infection of Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) in Compatible Wheat
The Plant Pathology Journal
2021 .10
-
Carboxymethylated chitosan alleviated oxidative stress injury in retinal ganglion cells via IncRNA-THOR/IGF2BP1 axis
Genes & Genomics
2021 .01
-
Dipeptidyl peptidase 4 as an injury-responsive protein in the mouse sciatic nerve
Molecules and Cells
2024 .01
-
N-Acetyl-D-Glucosamine Kinase Promotes the Axonal Growth of Developing Neurons
Molecules and Cells
2015 .10
-
New insights into the evolutionary history of resistance gene candidates in coconut palms and their expression profiles in palms affected by lethal yellowing disease
Genes & Genomics
2016 .01
-
xCyp26c Induced by Inhibition of BMP Signaling Is Involved in Anterior-Posterior Neural Patterning of Xenopus laevis
Molecules and Cells
2016 .04
-
Zebrafish is a central model to dissect the peripheral neuropathy
Genes & Genomics
2019 .01
-
Imaging neuronal cell with atomic force microscopy
한국진공학회 학술발표회초록집
2016 .08
-
Actin Depolymerizing Factor Destrin Regulates Cilia Development and Function during Vertebrate Embryogenesis
발생과 생식
2024 .09
-
Identification of pathways and genes associated with cerebral palsy
Genes & Genomics
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0