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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 141 - 144 (4page)
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초록· 키워드
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Dual specificity protein phosphatases (DUSPs) belong to the protein tyrosine phosphatase (PTP) family. DUSPsdephosphorylate both phospho-serine/threonine and phospho-tyrosine of mitogen activated protein kinases (MAPKs)and play important roles in cell growth, regulation and signaling. DUSP28, a member of the atypical DUSPs, hasdephosphorylation activity towards proteins involved in cellular signaling processes. DUSP28 is also implicated in thedevelopment of pancreatic cancer and liver cancer. The atomic resolution structure of DUSP28 should help the structurebaseddesign of specific and potent therapeutics. However, the structure and detailed function of DUSP28 have not beenelucidated yet. Here, we prepared a large quantity of DUSP28 protein and crystallized the protein. During the proteinpreparation, we encountered an unexpected proteolytic cleavage in the middle of the protein domain and overcamethe problem by identifying and mutating the pseudo-thrombin cleavage site. By using the purified protein, we were ableto grow diffraction quality crystals and collected a 2.1 A resolution diffraction data. The preliminary diffraction analysisrevealed that the crystal is in the space group P3121 with unit cell parameters of a = 78.85 A, b = 78.85 A, c = 90.26 A, α =90.00°, β = 90.00° and γ = 120.00°
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참고문헌 (17)
참고문헌 신청
Alonso, A / 2016 / The extended human PTPome : a growing tyrosine phosphatase family / FEBS J 283:2197~2201
Alonso, A / 2004 / Protein tyrosine phosphatases in the human genome / Cell 117:699~711
Bermudez, O / 2010 / The dual-specificity MAP kinase phosphatases: critical roles in development and cancer / Am J Physiol Cell Physiol 299:C189~C202
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Guan, K. L / 1991 / Eukaryotic proteins expressed in Escherichia coli : an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase / Anal Biochem 192:262~267
Alonso, A / 2004 / Protein tyrosine phosphatases in the human genome / Cell 117:699~711
Bermudez, O / 2010 / The dual-specificity MAP kinase phosphatases: critical roles in development and cancer / Am J Physiol Cell Physiol 299:C189~C202
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Guan, K. L / 1991 / Eukaryotic proteins expressed in Escherichia coli : an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase / Anal Biochem 192:262~267
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