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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.4
- 수록면
- 127 - 137 (11page)
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To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis.
These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.
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참고문헌 (49)
참고문헌 신청
Abremski K / 1984 / Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein / J Biol Chem 259:1509~1514
Akbudak MA / 2011 / Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice / Mol Biotech 49:82~89
Austin S / 1981 / A novel role for site-specific recombination in maintenance of bacterial replicons / Cell 25:729~736
Chong-Perez B / 2012 / Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system / J Biotechnol 159:265~273
Coppoolse ER / 2003 / Cre recombinase expression can result in phenotypic aberrations in plants / Plant Mol Biol 51:263~279
Akbudak MA / 2011 / Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice / Mol Biotech 49:82~89
Austin S / 1981 / A novel role for site-specific recombination in maintenance of bacterial replicons / Cell 25:729~736
Chong-Perez B / 2012 / Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system / J Biotechnol 159:265~273
Coppoolse ER / 2003 / Cre recombinase expression can result in phenotypic aberrations in plants / Plant Mol Biol 51:263~279
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