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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.13 No.3
- 발행연도
- 2008.9
- 수록면
- 196 - 203 (8page)
이용수
초록· 키워드
오류제보하기
Hwangtae, dried Alaska pollack, is a major storage product in the fish processing industry. Hwangtae is prepared by removing the internal organs and drying outdoors during the cold witner months by allowing it to thaw during the daytime and re-freeze at night under sub-zero (-10oC) conditions and gradually dry from December until the next April for around 5 months from Myungtae. In this study, ground Hwangtae was hydrolyzed using two proteolytic enzymes (pepsin and alcalase) which produced five soluble active peptides from Hwangtae (yellowish dried Pollack, Theragra chalcogramma) protein. Two different peptides with strong antioxidative activity were isolated from the hydrolysate using consecutive chromatographic methods of Sephadex G-25 gel, ion-exchange chromatography on a Sepharose-Sephadex C-25 gel, and high-performance liquid chromatography. The isolated peptides, APO1 and APO2, were composed of 16 and 13 amino acid residues, respectively. Both peptides contained a Gly residue at the C-terminus and the repeating motif Gly-Pro-Hyp. The peptide with a molecular weight less than 1,000 Daltons (APACE) obtained from enzymatic hydrolysates of Hwangtae exhibited the highest ACE inhibitory activity. The APACE peptides was composed of 4 amino acid residues (Gly-Leu-Leu-Pro). These results suggest that Hwangtae hydrolysates could be a good source of peptides with ACE inhibitory activity. Biochemical analysis indicated that two 70 kDa peptides (APG1 and APG2) isolated from the hydrolysate had gelatinoytic activity, which was shown to be a calcium dependent protease type as showed by gelatin SDS PAGE.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENT
REFERENCES
참고문헌 (39)
참고문헌 신청
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2. [학술지(정기간행물)] - Iwaniak A / 2007 / Protein as the source of physiologically and functionally active peptides / Acta Sci Pol Tehcnol Aliment 6 : 5 ~ 15
3. [학술지(정기간행물)] - Korhonen H / 2003 / Food-derived bioactive peptides: Opportunities for designing future foods / Curr Pharm Des 9 : 1297 ~ 1308
4. [학술지(정기간행물)] - Branen AL / 1975 / Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene / J Am Oil Chem Soc 52 : 59 ~ 63
5. [학술지(정기간행물)] - Osawa T / 1985 / Natural antioxidants isolated from Eucalyptus leaf waxes / J Agric Food Chem 33 : 777 ~ 780
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