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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.15 No.4
- 발행연도
- 2010.12
- 수록면
- 316 - 321 (6page)
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초록· 키워드
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Krill byproduct was hydrolyzed with Alcalase 2.4L to produce functional ingredients for high antioxidative activities against 1,1-dimethyl-2-picryl-hydrazyl (DPPH) radical and Fe. The objective of this study was to investigate the optimum condition for degree of hydrolysis and antioxidative activity of enzymatic hydrolysate produced with the commercial Alcalase using response surface methodology (RSM) with a central composite rotatable design (CCRD). The ranges of independent variables were pH 7.6～10.4 for initial pH and 50.9～79.1℃ for hydrolysis temperature and their dependent variables were degree of hydrolysis, Brix, amount of phenolic compounds, DPPHscavenging activity and Fe-chelating activity. RSM with CCRD was well designed to investigate the optimum condition for functional ingredients with high antioxidative activities using Alcalase 2.4L because of their high R² values of the range of 0.93～0.99 except the R² value of 0.50 for the amount of total phenolic compounds. The optimum hydrolysis conditions were pH 9.5 and 62℃ for degree of hydrolysis (DH) and pH 9.1 and 64℃ for DPPH-scavenging activity by response surface methodology. The yield of DH and DPPH-scavenging activity were 14.1±0.5% and 10.5±0.2%, respectively. It is advantageous to determine the optimum hydrolysis conditions of krill and its by-products for the creation of different kinds of food products, as well as to increase the usage of marine protein sources.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
ACKNOWLEDGEMENT
REFERENCES
참고문헌 (17)
참고문헌 신청
Robin MR / 1988 / Euphausia super－A critical review of estimates of annual production / Comp Biochem Physiol (90B):499~505
Suh HL / 1988 / Review on the reproduction feeding and longevity of the antarctic krill, Euphausia super / Bull Korean Fish Soc (21):303~310
Guerard F / 2002 / Production of tuna waste hydrolysates by a commercial neutral protease preparation / J Mol Catal B Enzym (19):489~498
Kim E / 1996 / Development of functional seasoning agents from skipjack preparation by-product with commercial proteases: I. Processing of hydrolysate from skipjack processing by-products with protease treatment / J Korean Soc Food Sci Nutr (25):608~616
Benjakul S / 1997 / Protein hydrolysates form Pacific whiting solid wastes / J Agric Food Chem (45):3423~3430
Suh HL / 1988 / Review on the reproduction feeding and longevity of the antarctic krill, Euphausia super / Bull Korean Fish Soc (21):303~310
Guerard F / 2002 / Production of tuna waste hydrolysates by a commercial neutral protease preparation / J Mol Catal B Enzym (19):489~498
Kim E / 1996 / Development of functional seasoning agents from skipjack preparation by-product with commercial proteases: I. Processing of hydrolysate from skipjack processing by-products with protease treatment / J Korean Soc Food Sci Nutr (25):608~616
Benjakul S / 1997 / Protein hydrolysates form Pacific whiting solid wastes / J Agric Food Chem (45):3423~3430
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UCI(KEPA) : I410-ECN-0101-2012-511-003794875