지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 78 - 84 (7page)
이용수
초록· 키워드
오류제보하기
Background: In the present study, metabolite profiles of ginsenosides Rk1 and Rg5 from red ginseng or red notoginseng in zebrafish were qualitatively analyzed with ultraperformance liquid chromatography/quadrupoleetime-of-flight MS, and the possible metabolic were pathways proposed.
Methods: After exposing to zebrafish for 24 h, we determined the metabolites of ginsenosides Rk1 and Rg5. The chromatography was accomplished on UPLC BEH C18 column using a binary gradient elution of 0.1% formic acetonitrilee0.1% formic acid water. The quasimolecular ions of compounds were analyzed in the negative mode. With reference to quasimolecular ions and MS2 spectra, by comparing with reference standards and matching the empirical molecular formula with that of known published compounds, and then the potential structures of metabolites of ginsenosides Rk1 and Rg5 were acquired.
Results: Four and seven metabolites of ginsenoside Rk1 and ginsenoside Rg5, respectively, were identified in zebrafish. The mechanisms involved were further deduced to be desugarization, glucuronidation, sulfation, and dehydroxymethylation pathways. Dehydroxylation and loss of C-17 residue were also metabolic pathways of ginsenoside Rg5 in zebrafish.
Conclusion: Loss of glucose at position C-3 and glucuronidation at position C-12 in zebrafish were regarded as the primary physiological processes of ginsenosides Rk1 and Rg5.
Methods: After exposing to zebrafish for 24 h, we determined the metabolites of ginsenosides Rk1 and Rg5. The chromatography was accomplished on UPLC BEH C18 column using a binary gradient elution of 0.1% formic acetonitrilee0.1% formic acid water. The quasimolecular ions of compounds were analyzed in the negative mode. With reference to quasimolecular ions and MS2 spectra, by comparing with reference standards and matching the empirical molecular formula with that of known published compounds, and then the potential structures of metabolites of ginsenosides Rk1 and Rg5 were acquired.
Results: Four and seven metabolites of ginsenoside Rk1 and ginsenoside Rg5, respectively, were identified in zebrafish. The mechanisms involved were further deduced to be desugarization, glucuronidation, sulfation, and dehydroxymethylation pathways. Dehydroxylation and loss of C-17 residue were also metabolic pathways of ginsenoside Rg5 in zebrafish.
Conclusion: Loss of glucose at position C-3 and glucuronidation at position C-12 in zebrafish were regarded as the primary physiological processes of ginsenosides Rk1 and Rg5.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)] - Mi Ra Lee / 2012 / Comparative Study of White and Steamed Black Panax ginseng, P. quinquefolium, and P. notoginseng on Cholinesterase Inhibitory and Antioxidative Activity / Journal of Ginseng Research 36 (1) : 93 ~ 101
[학술지(정기간행물)] - Dong TT / 2003 / Chemical assessment of roots of Panax notoginseng in China : regional and seasonal variations in its active constituents / J Agric Food Chem 51 : 4617 ~ 4623
[학술지(정기간행물)] - Chan EC / 2007 / Ultra-performance liquid chromatography/time-of-flight mass spectrometry based metabolomics of raw and steamed Panax notoginseng / Rapid Commun Mass Spectrom 21 : 519 ~ 528
[학술지(정기간행물)] - Chen B / 2014 / Simultaneous determination of ten active ginsenosides in steamed notoginseng by UPLC / Chin J Chin Material Media 39 : 1614 ~ 1619
[학술지(정기간행물)] - Sun S / 2010 / Effects of steaming the root of Panax notoginseng on chemical composition and anticancer activities / Food Chem 118 : 307 ~ 314
[학술지(정기간행물)] - Dong TT / 2003 / Chemical assessment of roots of Panax notoginseng in China : regional and seasonal variations in its active constituents / J Agric Food Chem 51 : 4617 ~ 4623
[학술지(정기간행물)] - Chan EC / 2007 / Ultra-performance liquid chromatography/time-of-flight mass spectrometry based metabolomics of raw and steamed Panax notoginseng / Rapid Commun Mass Spectrom 21 : 519 ~ 528
[학술지(정기간행물)] - Chen B / 2014 / Simultaneous determination of ten active ginsenosides in steamed notoginseng by UPLC / Chin J Chin Material Media 39 : 1614 ~ 1619
[학술지(정기간행물)] - Sun S / 2010 / Effects of steaming the root of Panax notoginseng on chemical composition and anticancer activities / Food Chem 118 : 307 ~ 314
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UCI(KEPA) : I410-ECN-0101-2018-524-001598714