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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 180 - 187 (8page)
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초록· 키워드
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Background: The incidence of halitosis has a prevalence of 22-50% throughout the world and is generally caused by anaerobic oral microorganisms, such as Fusobacterium nucleatum, Clostridium perfringens, and Porphyromonas gingivalis. Previous investigations on the structure-activity relationships of ginsenosides have led to contrasting results. Particularly, the antibacterial activity of less polar ginsenosides against halitosis-related bacteria has not been reported.
Methods: Crude saponins extracted from the Panax quinquefolius leaf-stem (AGS) were treated at 130℃ for 3 h to obtain heat-transformed saponins (HTS). Five ginsenoside-enriched fractions (HTS-1, HTS-2, HTS-3, HTS-4, and HTS-5) and less polar ginsenosides were separated by HP-20 resin absorption and HPLC, and the antimicrobial activity and mechanism were investigated.
Results: HPLC with diode-array detection analysis revealed that heat treatment induced an extensive conversion of polar ginsenosides (-Rg1/Re, -Rc, -Rb2, and -Rd) to less polar compounds (-Rg2, -Rg3, -Rg6, -F4, -Rg5, and -Rk1). The antimicrobial assays showed that HTS, HTS-3, and HTS-4 were effective at inhibiting the growth of F. nucleatum, C. perfringens, and P. gingivalis. Ginsenosides-Rg5 showed the best antimicrobial activity against the three bacteria, with the lowest values of minimum inhibitory concentration and minimum bactericidal concentration. One major reason for this result is that less polar ginsenosides can more easily damage membrane integrity.
Conclusion: The results indicated that the less polar ginsenoside-enriched fraction from heat transformation can be used as an antibacterial agent to control halitosis.
Methods: Crude saponins extracted from the Panax quinquefolius leaf-stem (AGS) were treated at 130℃ for 3 h to obtain heat-transformed saponins (HTS). Five ginsenoside-enriched fractions (HTS-1, HTS-2, HTS-3, HTS-4, and HTS-5) and less polar ginsenosides were separated by HP-20 resin absorption and HPLC, and the antimicrobial activity and mechanism were investigated.
Results: HPLC with diode-array detection analysis revealed that heat treatment induced an extensive conversion of polar ginsenosides (-Rg1/Re, -Rc, -Rb2, and -Rd) to less polar compounds (-Rg2, -Rg3, -Rg6, -F4, -Rg5, and -Rk1). The antimicrobial assays showed that HTS, HTS-3, and HTS-4 were effective at inhibiting the growth of F. nucleatum, C. perfringens, and P. gingivalis. Ginsenosides-Rg5 showed the best antimicrobial activity against the three bacteria, with the lowest values of minimum inhibitory concentration and minimum bactericidal concentration. One major reason for this result is that less polar ginsenosides can more easily damage membrane integrity.
Conclusion: The results indicated that the less polar ginsenoside-enriched fraction from heat transformation can be used as an antibacterial agent to control halitosis.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusions
References
참고문헌 (39)
참고문헌 신청
[학술지(정기간행물)] - Yang WZ / 2014 / Saponins in the genus Panax L. (Araliaceae): a systematic review of their chemical diversity / Phytochemistry 106 : 7 ~ 24
[학술지(정기간행물)] - Xue Y / 2009 / RP-HPLC determination of twelve ginsenosides in extract of root and stem leaf from Panax quinquefolius L / Chin J Pharmaceut Ana 29 : 79 ~ 81
[학술지(정기간행물)] - Li FL / 2013 / Research Progress of Pharmacology effect about ginsenosides from stems and leaves of Panax ginseng / Guizhou Agric Sci 41 : 54 ~ 57
[학술지(정기간행물)] - Bai-Shen Sun / 2011 / Repetitious Steaming-induced Chemical Transformations and Global Quality of Black Ginseng Derived from Panax ginseng by HPLC-ESI- MS/MS^n Based Chemical Profiling Approach / Biotechnology and Bioprocess Engineering 16 (5) : 956 ~ 965
[학술지(정기간행물)] - Kim WY / 2000 / Steaming of ginseng at high temperature enhances biological activity / J Nat Prod 63 : 1702 ~ 1704
[학술지(정기간행물)] - Xue Y / 2009 / RP-HPLC determination of twelve ginsenosides in extract of root and stem leaf from Panax quinquefolius L / Chin J Pharmaceut Ana 29 : 79 ~ 81
[학술지(정기간행물)] - Li FL / 2013 / Research Progress of Pharmacology effect about ginsenosides from stems and leaves of Panax ginseng / Guizhou Agric Sci 41 : 54 ~ 57
[학술지(정기간행물)] - Bai-Shen Sun / 2011 / Repetitious Steaming-induced Chemical Transformations and Global Quality of Black Ginseng Derived from Panax ginseng by HPLC-ESI- MS/MS^n Based Chemical Profiling Approach / Biotechnology and Bioprocess Engineering 16 (5) : 956 ~ 965
[학술지(정기간행물)] - Kim WY / 2000 / Steaming of ginseng at high temperature enhances biological activity / J Nat Prod 63 : 1702 ~ 1704
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UCI(KEPA) : I410-ECN-0101-2018-524-001598830