지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The diagnosis of atopic dermatitis (AD) includes a test that checks allergen-mediated skin reactions and a method of measuring the total IgE and allergen-specific IgE in blood. However, these test methods are performed directly on the patient, which cause some pain or discomfort. In addition, the skin response test or IgE may result in false negative in about 20% of patients. In the present study, to identify specific biomarkers, HaCaT cells were used as a human keratinocyte that make up the skin, were treated IL-4 and IL-13 for 24 hours to induce a situation similar to keratinocytes in AD patients. In the HaCaT cells, pro-inflammatory cytokine such as IL-5, IL-6, and MCP-1 were increased by IL-4 and IL-13 and skin barrier proteins was reduced by IL-4 and L-13. This results showed that a situation similar to the stratum corneum of an actual patient is induced in HaCaT cells. And then the secretions of Kallikrein (KLK) 5 and KLK7 protease were checked by enzyme-linked immunosorbent assay (ELISA). It was specifically increased by IL-4 and IL-13. This showed that AD-related protease can be detected at the protein level using keratinocytes that can be taken in a non-invasive manner and suggested the possibility of applying it to AD diagnosis.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
Bennett K / 2010 / New role for LEKTI in skin barrier formation: label-free quantitative proteomic identification of caspase 14 as a novel target for the protease inhibitor LEKTI / Journal of Proteome Research 9:4289~4294
Boguniewicz M / 2011 / Atopic dermatitis: a disease of altered skin barrier and immune dysregulation / Immunological Reviews 242:233~246
Boguniewicz M / 2006 / Atopic dermatitis / Journal of Allergy and Clinical Immunology 118:40~43
Cork MJ / 2009 / Epidermal barrier dysfunction in atopic dermatitis / Journal of Investigative Dermatology 129:1892~1908
Di ZH / 2016 / T Helper 1 and T Helper 2 Cytokines Differentially Modulate Expression of Filaggrin and its Processing Proteases in Human Keratinocytes / Chinese Medical Journal 129:295~303
Boguniewicz M / 2011 / Atopic dermatitis: a disease of altered skin barrier and immune dysregulation / Immunological Reviews 242:233~246
Boguniewicz M / 2006 / Atopic dermatitis / Journal of Allergy and Clinical Immunology 118:40~43
Cork MJ / 2009 / Epidermal barrier dysfunction in atopic dermatitis / Journal of Investigative Dermatology 129:1892~1908
Di ZH / 2016 / T Helper 1 and T Helper 2 Cytokines Differentially Modulate Expression of Filaggrin and its Processing Proteases in Human Keratinocytes / Chinese Medical Journal 129:295~303
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UCI(KEPA) : I410-ECN-0101-2019-510-000546472