지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 291 - 299 (9page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenosides of Korean Red Ginseng extracts (RGE) and its saponin components suppress secretion of inflammasome-mediating cytokines, whereas the nonsaponin fraction (NS) of RGE oppositely stimulates cytokine secretion. Although direct exposure of NS to macrophages in mice induces cytokine production, oral administration of NS has not been studied in inflammasome-related disease in animal models.
Methods: Mice were fed RGE or NS for 7 days and then developed peritonitis. Peritoneal cytokines were measured, and peritoneal exudate cells (PECs) were collected to assay expression levels of a set of tolllike receptors (TLRs) and cytokines in response to NS ingestion. In addition, the role of intestinal bacteria in NS-fed mice was assessed. The effect of preexposure to NS in bone marrowederived macrophages (BMDMs) on cytokine production was further confirmed.
Results: NS ingestion attenuated secretion of peritoneal cytokines resulting from peritonitis. In addition, the isolated PECs from NS-fed mice presented lower TLR transcription levels than PECs from control diet efed mice. BMDMs treated with NS showed downregulation of TLR4 mRNA and protein expression, which was mediated by the TLR4-MyD88-NFkB signal pathway. BMDMs pretreated with NS produced less cytokines in response to TLR4 ligands.
Conclusion: NS administration directly inhibits TLR4 expression in inflammatory cells such as macrophages, thereby reducing secretion of cytokines during peritonitis.
Methods: Mice were fed RGE or NS for 7 days and then developed peritonitis. Peritoneal cytokines were measured, and peritoneal exudate cells (PECs) were collected to assay expression levels of a set of tolllike receptors (TLRs) and cytokines in response to NS ingestion. In addition, the role of intestinal bacteria in NS-fed mice was assessed. The effect of preexposure to NS in bone marrowederived macrophages (BMDMs) on cytokine production was further confirmed.
Results: NS ingestion attenuated secretion of peritoneal cytokines resulting from peritonitis. In addition, the isolated PECs from NS-fed mice presented lower TLR transcription levels than PECs from control diet efed mice. BMDMs treated with NS showed downregulation of TLR4 mRNA and protein expression, which was mediated by the TLR4-MyD88-NFkB signal pathway. BMDMs pretreated with NS produced less cytokines in response to TLR4 ligands.
Conclusion: NS administration directly inhibits TLR4 expression in inflammatory cells such as macrophages, thereby reducing secretion of cytokines during peritonitis.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (31)
참고문헌 신청
Ru W / 2015 / Chemical constituents and bioactivities of Panax ginseng (C. A. Mey.) / Drug Discov Ther 9:23~32
Seung-Hoon Baek / 2012 / Recent Methodology in Ginseng Analysis / Journal of Ginseng Research 36(2):119~134
손은화 / 2011 / Effects of Korean Red Ginseng Extract for the Treatment of Atopic Dermatitis-Like Skin Lesions in Mice / Journal of Ginseng Research 35(4):479~486
Kim J / 2014 / Korean red ginseng extracts inhibit NLRP3 and AIM2 inflammasome activation / Immunol Lett 158:143~150
기지예 / 2017 / Korean Red Ginseng improves atopic dermatitis-like skin lesions by suppressing expression of proinflammatory cytokines and chemokines in vivo and in vitro / Journal of Ginseng Research 41(2):134~143
Seung-Hoon Baek / 2012 / Recent Methodology in Ginseng Analysis / Journal of Ginseng Research 36(2):119~134
손은화 / 2011 / Effects of Korean Red Ginseng Extract for the Treatment of Atopic Dermatitis-Like Skin Lesions in Mice / Journal of Ginseng Research 35(4):479~486
Kim J / 2014 / Korean red ginseng extracts inhibit NLRP3 and AIM2 inflammasome activation / Immunol Lett 158:143~150
기지예 / 2017 / Korean Red Ginseng improves atopic dermatitis-like skin lesions by suppressing expression of proinflammatory cytokines and chemokines in vivo and in vitro / Journal of Ginseng Research 41(2):134~143
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