지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 323 - 329 (7page)
이용수
초록· 키워드
오류제보하기
Transgene expression was analyzed in tomato plants. Four lines of neomycin phosphotransferase II gene (NPTII) and the trehalose biosynthetic fusion gene (TPSP) transformed T0 plants showed kanamycin resistance on selection medium. However, the analysis of phenotype (kanamycin resistance) and mRNA expression in T1 plants indicated that the expression of the NPTII and TPSP transgenes was down-regulated to an undetectable level in two independent lines 1 and 11. Southern analysis demonstrated that the lines 1 and 11 had multicopies of the transgenes, whereas the typical transgenic lines 2 and 10 had 1 or 2 copies. DNA methylation analysis using methylation sensitive enzyme detected accumulated CpG DNA methylation on TPSP coding region and CaMV35S promoter region in the line 11, but not the typical transgenic line 2. These results suggest that multicopy transgene in plants is attributed to down-regulation of the transgene expression via transcriptional gene silencing.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Concatemer-Associated Transgene Expression Patterns in Transgenic Marine Medaka Oryzias dancena Strains
Fisheries and Aquatic Sciences
2015 .01
-
Comparative Analysis of Transgene Copy Numbers and Expression Characteristics across Multiple Transgenic Marine Medaka Oryzias dancena Strains carrying the β-Actin Promoter-Driven GFP Reporter
Fisheries and Aquatic Sciences
2015 .01
-
Expression of the Transgene is Consistently Inherited to High Numbers of Generations and Independent on Its Source
한국동물번식학회지
2008 .01
-
DNA methyltransferase 3a is Correlated with Transgene Expression in Transgenic Quails
한국축산학회지
2011 .01
-
Methylation Pattern Analysis of Double-Transformed Tobacco Plants with Antisense-Oriented MET1 Gene
HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY
2006 .12
-
Unexpected Promoter Methylation Occurred by Homology Dependent Gene Silencing in Transgenic Tobacco
한국원예학회 학술발표요지
2011 .10
-
무선발 형질전환 Bt벼의 도입유전자 구조 분석
Journal of Plant Biotechnology
2013 .01
-
이중형질전환 담배에서의 methylation pattern 분석
한국원예학회 학술발표요지
2005 .10
-
Inheritance and expression of transgene in SOD2-Transgenic petunia descendants and their morphological traits
Journal of Plant Biotechnology
2009 .01
-
[S3-1] Nutrients and DNA Methylation
한국식품영양과학회 학술대회발표집
2007 .10
-
Organ Specific Expression of the nos-NPT II Gene in Transgenic Hybrid Poplar
한국산림과학회지
1995 .01
-
Production of Transgenic Sweetpotato (Ipomoea batatas (L.) Lam.) Lines via Microprojectile Bombardment
한국육종학회지
2005 .01
-
Promoter Methylation Analysis of T1 Generation of GFP Transgenic Tobacco
한국원예학회 학술발표요지
2011 .10
-
Analysis of DNA methylation and gene expression in chicken primordial germ cells
한국가금학회 정기총회 및 학술발표회
2013 .11
-
Instability of Transgene Expression in Transgenic Tobacco
한국원예학회 기타 간행물
2006 .08
-
Development of transgenic cucumber expressing TPSP gene and morphological alterations
Journal of Plant Biotechnology
2010 .01
-
Alteration of DNA Methylation in Oct-4 Gene in Mouse Preimplantation Embryos by the Interference RNA
한국동물번식학회지
2007 .01
-
Promoter Methylation Analysis of Regenerated GFP Transgenic Tobacco
한국원예학회 학술발표요지
2011 .05
-
MdMADS2–transgenic chrysanthemum (Dendranthema grandiflorum(Ramat.) Kitamura) showing the reduction of the days to flowering
Journal of Plant Biotechnology
2009 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0