지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 441 - 449 (9page)
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초록· 키워드
오류제보하기
Background: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that included bacteria and other microorganisms. The NGS results were compared with the results of other assays, and NGS was evaluated for its feasibility for predicting vaginitis.
Methods: In total, 89 vaginal swab specimens were collected. Microscopic examinations of Gram staining and microbiological cultures were conducted on 67 specimens. NGS was performed with GS junior system on all of the vaginal specimens for the 16S rRNA, internal transcribed spacer (ITS), and Tvk genes to detect bacteria, fungi, and Trichomonas vaginalis. In addition, DNA probe assays of the Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis were performed. Various predictors of diversity that were obtained from the NGS data were analyzed to predict vaginitis.
Results: ITS sequences were obtained in most of the specimens (56.2%). The compositions of the intermediate and vaginitis Nugent score groups were similar to each other but differed from the composition of the normal score group. The fraction of the Lactobacillus spp. showed the highest area under the curve value (0.8559) in ROC curve analysis. The NGS and DNA probe assay results showed good agreement (range, 86.2-89.7%).
Conclusions: Fungi as well as bacteria should be considered for the investigation of vaginal microbiome. The intermediate and vaginitis Nugent score groups were indistinguishable in NGS. NGS is a promising diagnostic tool of the vaginal microbiome and vaginitis, although some problems need to be resolved.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Hummelen R / 2010 / Deep sequencing of the vaginal microbiota of women with HIV / PLoS One 5:e12078
Schellenberg JJ / 2011 / Molecular definition of vaginal microbiota in East African commercial sex workers / Appl Environ Microbiol 77:4066~4074
Ling Z / 2010 / Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis / BMC Genomics 11:488
Liu MB / 2013 / Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis / PLoS One 8:e79812
Ravel J / 2011 / Vaginal microbiome of reproductive-age women / Proc Natl Acad Sci U S A 108(S1):4680~4687
Schellenberg JJ / 2011 / Molecular definition of vaginal microbiota in East African commercial sex workers / Appl Environ Microbiol 77:4066~4074
Ling Z / 2010 / Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis / BMC Genomics 11:488
Liu MB / 2013 / Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis / PLoS One 8:e79812
Ravel J / 2011 / Vaginal microbiome of reproductive-age women / Proc Natl Acad Sci U S A 108(S1):4680~4687
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